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Thromb Haemost 1983; 50(02): 595-600
DOI: 10.1055/s-0038-1665264
Original Article
Schattauer GmbH Stuttgart

Enhancement of the Release Reaction Not Accompanied with Enhanced Phosphatidylinositol Turnover in the Reserpinized Rabbit Blood Platelets

Y Watanabe
The Department of Physiological Chemistry, College of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
,
M Soda
The Department of Physiological Chemistry, College of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
,
N Fukamachi
The Department of Physiological Chemistry, College of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
,
B Kobayashi
The Department of Physiological Chemistry, College of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

Received 21 March 1983

Accepted 27 June 1983

Publication Date:
18 July 2018 (online)

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Summary

Thrombin-induced platelet release reaction examined with secretion of calcium and N-acetylglucosaminidase was significantly enhanced in the platelets from reserpine-treated rabbits as compared with the control. On the other hand, 32P-incorporation into phosphatidic acid was suppressed in the reserpinized platelets in activated state. Thrombin induced phosphatidylinositol (PI)- breakdown, which was examined by decreases in radioactivity and content of PI, and an increase in diacylglycerol, was not enhanced in the reserpinized platelets as compared with the control. The phosphorylation of the specific protein coupled to thrombin- induced platelet PI-breakdown was not stimulated in the reserpinized platelets as compared with the control. In contrast to PI, PC-degradation by thrombin was significantly stimulated in the reserpinized platelets. Possible existence of pathway(s) other than that associated with an enhancement of Pl-tumover is conceivable as a mechanism involved in platelet release reaction.

Keywords

Platelet release reaction - Reserpine - Phosphatidylinositol response - Thrombin
 

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