Verapamil and Collagen-Induced Platelet Reactions- Evidence for a Role for Intracellular Calcium in Platelet Activation

P Han; C Boatwright; N G Ardlie

doi:10.1055/s-0038-1665249

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1983; 50(02): 537-540
DOI: 10.1055/s-0038-1665249

Original Article

Schattauer GmbH Stuttgart

Verapamil and Collagen-Induced Platelet Reactions- Evidence for a Role for Intracellular Calcium in Platelet Activation

P Han

The Department of Medicine and Clinical Science, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia

,

C Boatwright

The Department of Medicine and Clinical Science, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia

,

N G Ardlie

The Department of Medicine and Clinical Science, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia

› Author Affiliations

Abstract

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Summary

Calcium is considered to have an essential role in various platelet reactions. Using platelets preincubated with Chlortetracycline, a fluorescent divalent cation indicator, and suspended in a calcium free medium, it was shown that collagen-induced intracellular calcium redistribution occurred before the platelet shape change, the release reaction and thromboxane B₂ formation. Verapamil, at concentrations which affect intracellular calcium movements, inhibited intracellular calcium redistribution in platelets and the subsequent collagen-induced platelet reactions. Low concentrations of the ionophore A23187 overcame the inhibitory effect of verapamil. These experiments provide evidence that intracellular calcium mobilization is involved in the activation of platelets by collagen. Furthermore, calcium may be released from different cellular pools since platelet secretion, aggregation and thromboxane B₂ formation were inhibited at lower concentrations of verapamil than was the platelet shape change.

Keywords

Platelet activation - Collagen - Verapamil - A23187 - Chlortetracycline - Calcium mobilization

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References

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