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Thromb Haemost 1983; 50(02): 527-529
DOI: 10.1055/s-0038-1665246
Original Article
Schattauer GmbH Stuttgart

The Ability of Fibrinogens, FI and FII, to Support ADP- Induced Platelet Aggregation

H M Phillips
Departments of Biochemistry and Medicine, University of Arkansas for Medical Sciences, and Veterans Administration Medical Center, Little Rock, Arkansas, U.S.A.
,
A Mansouri
Departments of Biochemistry and Medicine, University of Arkansas for Medical Sciences, and Veterans Administration Medical Center, Little Rock, Arkansas, U.S.A.
,
C A Perry
Departments of Biochemistry and Medicine, University of Arkansas for Medical Sciences, and Veterans Administration Medical Center, Little Rock, Arkansas, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 20 December 1982

Accepted 18 May 1983

Publication Date:
18 July 2018 (online)

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Summary

Fibrinogen plays an integral part in ADP-induced platelet aggregation. Controversy exists in regard to the role of the carboxy termini of fibrinogen Aa chains in this reaction. We have attempted to clarify this problem in view of the availability of a highly purified FII fibrinogen fraction. Kabi fibrinogen or its purified fractions FI, FII and FIII-IV-V were added to washed platelets in the presence of Tyrode-HEPES buffer pH 7.4. Aggregation was initiated by the addition of calcium and ADP. These fibrinogen fractions equally promoted ADP-induced platelet aggregation. The major difference among these fractions is in their Aα chains. The FI fraction contains intact Aα chains while FII and FIH-IV-V fractions have one and two partially degraded Aα chains at the carboxy terminal portion respectively. We conclude that the carboxy terminal portion of the Aα chain does not play an important role in promoting ADP-induced platelet aggregation.

Keywords

Fibrinogen - Platelet aggregation - Fibrinogen fractions
 

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