Thromb Haemost 1983; 50(02): 509-512
DOI: 10.1055/s-0038-1665242
Original Article
Schattauer GmbH Stuttgart

Gel Filtration Patterns of Factor VIII Coagulant Antigen and Factor VIII Related Antigen in Normal and von Willebrand’s Disease

Juan Chediak
The Department of Haematology, University Hospital of Wales, Cardiff, Wales, U. K.
,
Ian Peake
The Department of Haematology, University Hospital of Wales, Cardiff, Wales, U. K.
,
Arthur Bloom
The Department of Haematology, University Hospital of Wales, Cardiff, Wales, U. K.
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Publikationsverlauf

Received 27. Oktober 1982

Accepted 07. Januar 1983

Publikationsdatum:
18. Juli 2018 (online)

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Summary

Gel filtration (sepharose 2B CL) patterns of factor VIII coagulant antigen (VIIIC :Ag) and factor VIII related antigen (VIIIR: Ag) were obtained using normal plasma and plasma from patients with von Willebrand’s disease. The latter group consisted of five individuals with normal mobility of factor VIIIR :Ag on cross-immunoelectrophoresis (Type I) and five others with abnormal (increased) mobility (Type II). Results showed that the elution of VIIIC: Ag was delayed in those subjects whose ratio of VIIIR :Ag to VIIIC :Ag was reduced. It has previously been reported that VIIIR :Ag exerts a stabilizing influence on the coagulant activity of factor VIII (VIII: C); our data suggests that when VIIIR :Ag is deficient, abnormal (low molecular weight) forms of VIIIC: Ag circulate.