Absence of Synergism Between Tissue-Type Plasminogen Activator (t-PA), Single-Chain UrokinaseType Plasminogen Activator (scu-PA) and Urokinase on Clot Lysis in a Plasma Milieu In Vitro

D Collen; F De Cock; E Demarsin; H R Lijnen; D C Stump

doi:10.1055/s-0038-1661598

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1986; 56(01): 035-039
DOI: 10.1055/s-0038-1661598

Original Article

Schattauer GmbH Stuttgart

Absence of Synergism Between Tissue-Type Plasminogen Activator (t-PA), Single-Chain UrokinaseType Plasminogen Activator (scu-PA) and Urokinase on Clot Lysis in a Plasma Milieu In Vitro

Authors

D Collen

The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
F De Cock

The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
E Demarsin

The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
H R Lijnen

The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
D C Stump

The Center for Thrombosis and Vascular Research, University of Leuven, Belgium

Abstract

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Summary

A potential synergic effect of tissue-type plasminogen activator (t-PA), single-chain urokinase-type plasminogen activator (scuPA) or urokinase on clot lysis was investigated in a whole human plasma system in vitro. The system consisted of a human plasma clot labeled with ¹²⁵I-fibrinogen, immersed in titrated whole human plasma, to which the thrombolytic agents were added. Clot lysis was quantitated by measurement of released ¹²⁵I, and activation of the fibrinolytic system in the surrounding plasma by measurements of fibrinogen and α₂-antiplasmin.

t-PA, scu-PA and urokinase induced a dose-dependent and time-dependent clot lysis; 50 percent lysis after 2 h was obtained with 5 nM t-PA, 20 nM scu-PA and 12 nM urokinase. At these concentrations no significant activation of the fibrinolytic system in the plasma was observed with t-PA and scu-PA, whereas urokinase caused significant α₂-antiplasmin consumption and concomitant fibrinogen degradation. The shape of the dose-response curves was different; t-PA and urokinase showed a log linear dose-response whereas that of scu-PA was sigmoidal.

Keywords

t-PA - scu-PA - Urokinase - Synergism - Thrombolysis

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References

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