Dysfunctional Plasminogen in Full Term Newborn - Study of Active Site of Plasmin

Summary

The functional activity and active site of plasmin in full-term newborns have been studied and compared to those in adults in order to investigate the nature of the abnormality found in newborn plasminogen descried in a previous paper.

The functional activity of newborn plasminogen measured on chromogenic substrate was approximately 18% that of adult plasminogen when streptokinase was used as an activator and 12% when urokinase was used.

Proteolysis of newborn plasminogen by urokinase yielding a two-chain plasmin form occurred normally, but the incorporation of diisopropylphosphorofluoridate into the light chain of newborn plasmin was approximately 23% of that observed in the light chain of adult plasmin.

These observations suggest that the abnormality of full-term newborn plasminogen is located in the active site of the molecule.

Key Words

Dysfunctional plasminogen - Full-term newborns - Active site of plasmin

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