Circulating Fibrinogen Degradation Products (FDP) in Dog Blood after Intravenous Thrombin Infusion

Summary

1. The effect of thrombin infusion into dogs was studied with the use of ¹³¹I-labelled fibrinogen and compared with the better known effect of plasmin.

2. Rapid infusion of thrombin brings about almost complete defibrination followed by the appearance of high molecular fragments of the fibrinogen molecule.

3. Some anticoagulant activity of plasma, but not as pronounced as after plasmin infusion, is observed in the case of thrombin infusion.

4. A hypothesis is discussed, according to which the proteolytic degradation of fibrin which is formed as a result of thrombin action, and cleared out of the circulating blood, does occur. The products are then released back into the circulation system.

• References

• 1 Astrup T, Müllertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Bio-chem. 40: 346 1952;
• 2 Blombach B, Blombach M. Purification of human and bovine fibrinogen. Arkiv for Kemi. iö: 415 1956
• 3 Budzynski A. Z, Lipinski B, Batallo Z. S. Unpublished results
• 4 Copley A. L. Studies on human placental thromboplastin in vitro and in vivo. Science 101: 436 1945;
• 5 Fulton L. D, Page E. W. Nature of the refractory state following sublethal closes of human placental thromboplastin. Proc. Soc. exp. Biol. 68: 594 1948;
• 6 Hardaway R. M. The role of intravascular clotting in the etiology of shock. Ann. Surg. 155: 325 1962;
• 7 Hardaway R, Brune W, Greever E, Burns J, Mock H. Studies on the role of intravascular coagulation in irreversible hemorrhagic shock. Ann. Surg. 155: 241 1962;
• 8 Hardaway R. M, Weiss F. H. Intracapillary clotting as the etiology of shock. Arch. Surg. 83: 851 1961;
• 9 Hardaway R. M, Watson H. E, Weiss F. H. Alterations in blood coagulation mechanism after intra-aortic injection of thrombin. Arch. Surg. 81: 983 1960;
• 10 Jürgens R, Studer A. Zur Wirkung des Thrombins. Helv. physiol. pharmacol. Acta 6: 134 1948;
• 11 Kekwick R. A, Mackay M. E, Nance M. H, Record B. R. The purification of human fibrinogen. Biochem. J. 60: 671 1955;
• 12 Kline D. L. The purification and cristallization of plasminogen (Profibrinolysin). J. Biol. Chem. 204: 949 1953;
• 13 Kotschy M. O. O aktywacji plazminy w krzepnacej krwi Postepy Hig. Med. dosw. 18: 45 1964;
• 14 Kowalski E, Budzynski A. Z, Kopec Maria, Batallo Z. S, Lipinski B, Wçgrzynowicz Z. Studies on the molecular pathology and pathogenesis of bleeding in severe fibrinolytic states in dogs. Thrombos. Diathes. haemorrh. (Stuttg.) 12: 69 1964;
• 15 Kowarzyk H, Kotschy M. Clot formation and fibrinolysis. Congress of Purkynie Czecoslovak. Med. Soc. Prague, 1962. (In press.)
• 16 Kudriaszow B. A, Ulytina P. D. Existence and significance of a physiological anticoagulat-ing system. Nature 182: 397 1958;
• 17 Kudriaszow B. A, Ulytina P. D. Interrelations between the blood coagulating system and the physiological anticoagulating system. Nature 184: 4684 1959;
• 18 Lipinski B, Budzynski A. Z, Batallo Z. S, Kowalski E. Isolation and characterisation of cold-insoluble fibrinogen complex from bovine plasma. Acta biochim. pol. 11: 527 1964;
• 19 Loewy A. G, Dunathan K, Kriel R, Wolfinger Jr H. L. Fibrinase I. Purification of substrate and enzyme. J. biol. Chem. 236: 2625 1961;
• 20 Mills G. A. The action of tissue extracts in the coagulation of blood. J. biol. Chem. 46: 167 1921;
• 21 Monkhouse F. G, Milojevie S. Changes in fibrinogen level after infusion of thrombin and thromboplastin. Amer. J. Physiol. 199: 1165 1960;
• 22 Niewiarowska M, Wçgrzynowicz Z. Influence of dicumarol derivatives on plasma fibrinolytic system. Thrombos. Diathes. haemorrh. (Stuttg.) 3: 297 1959;
• 23 Ratnojf O. D, Conley G. L. Studies on a fibrinogenemia. II. The defibrinating effect on dog blood of intravenous injection of thromboplastic material. Bull. Johns Hopk. Hosp. 88: 414 1951;
• 24 Sawyer W. D, Fletcher A. P, Alkjaersig N, Sherry S. Studies on the thrombolytic activity of human plasma. J. clin. Invest. 39: 426 1960;
• 25 Schneider C. L. The active principle of placental toxin: thromboplastin; its inactivation in blood: antithromboplastin. Amer. J. Physiol. 149: 123 1947;
• 26 Thomas L. Studies on the intravascular thromboplastic effect of tissue suspension in mice. I. The reaction of mice to intravenous injection of a sedimentable tissue component. Bull. Johns Hopk. Hosp. 81: 1 1947;