Collagen-Induced Platelet Aggregation as Studied by Electronic Particle Size Analysis

Summary

The collagen-induced response of free platelets during aggregation was measured with an electronic particle size analyzer. Aggregation was induced in platelet-rich plasma by 0.05 mg/mL collagen. The reaction was followed turbidimetrically and samples for electronic particle size analysis were obtained during aggregation.Free platelet number decreased rapidly after the addition of collagen. This decrease preceded detectable changes in transmittance and a significant reduction in free platelet number was measured during the classical lag phase. In addition, the size distribution of free platelets at variotrs times after the addition of collagen was not significantly altered indicating that large platelets are not preferentially involved in collagen-induced aggregation.

Keywords

Platelet aggregation - Collagen - Electronic particle Size analysis

• References

• 1 Zucker MB, Borrelli J. Platelet clumping produced by connective tissue suspensions and by collagen. Exp Biol Med 1962; 109: 779-787
• 2 Jamieson GA, Urban CL, Barber AJ. Enzymatic basis for platelet collagen adhesion as the primary step in hemostasis. Nature (New Biol) 1971; 234: 5-7
• 3 Santoro SA, Cunningham LW. Collagen-mediated platelet aggregation: The role of multiple interactions between the platelet surface and collagen. Thromb Haemostas 1980; 43: 158-162
• 4 Bensusan HB, Koh TL, Henry KG, Murray BA, Culp LA. Evidence that fibronectin is the collagen receptor on platelet membranes. Proc Nat Acad Sri USA 1978; 75: 5864-5868
• 5 Hovig T. Release of a platelet-aggregating substance (adenosine diphosphate) from rabbit blood platelets induced by saline »extract« of tendons. Thrombos Diathes Haemorrh 1963; 9: 264-278
• 6 Nunn B. Collagen-induced platelet aggregation: Evidence against the essential role of platelet adenosine diphosphate. Thromb Haemostas 1979; 42: 1193-1206
• 7 Packham MA, Kinlough-Rathbone RL, Reimers HJ, Scott S, Mustard JF. Mechanisms of platelet aggregation independent of adenosine diphosphate. In: Silver HJ, Smith JB, Kocsis JJ. (eds.) Prostaglandins in Hematology. Spectrum Publications, Inc; p 247 New York: 1977
• 8 Bora GV R. Quantitative investigations into the aggregation of blood platelets. J Physiol (Lond) 1962; 162: 67P-68P
• 9 Cronberg S. Evaluation of platelet aggregation. Coagulation 1970; 3: 139-151
• 10 Bom GV R, Hume M. Effects of numbers and sizes of platelet aggregates on the optical density of plasma. Nature (Lond) 1967; 215: 1027-1029
• 11 Nichols AR, Bosmann HB. Platelet aggregation: Newly quantified using non-empirical parameters. Thromb Haemostas 1979; 42: 679-693
• 12 McLean MR, Hause LL. Population dynamics during reversible platelet aggregation. American Association for the Advancement of Science Annual Meeting. 1980
• 13 McLean MR. Mechanisms of the Human Platelet Response to Aggregating Agents and to Heparin. Dissertation. The Medical College of Wisconsin 1981
• 14 Summerer MH, Genco PV, Katz AJ. The response of human platelets to hypotonic stress: Direct measurement of volume change. Ann Clin Lab Sri 1978; 8: 447-452
• 15 Mundschenk DD, Connelly DP, White JG, Brunning RD. An improved technique for the electronic measurement of platelet size and shape. J Lab Clin Med 1976; 88: 301-315
• 16 Paulus JM, Bury J, Grosdent JC. Control of platelet territory development in megakaryocytes. Blood Cells 1979; 5: 59-88
• 17 Karpatkin S. Heterogeneity of human platelets. VI. Correlation of platelet function with platelet volume Blood 1978; 51: 307-316