Download PDF
Thromb Haemost 1999; 82(05): 1412-1416
DOI: 10.1055/s-0037-1614846
Rapid Communications
Schattauer GmbH

Automated Latex Agglutination and ELISA Testing Yield Equivalent D-Dimer Results in Patients with Recent Myocardial Infarction

Victor J. Marder
1   From the Orthopaedic Hospital/UCLA School of Medicine, Los Angeles, CA
,
Wojciech Zareba
2   University of Rochester School of Medicine & Dentistry, Rochester, NY, USA
,
John T. Horan
2   University of Rochester School of Medicine & Dentistry, Rochester, NY, USA
,
Arthur J. Moss
2   University of Rochester School of Medicine & Dentistry, Rochester, NY, USA
,
Joel J. Kanouse
1   From the Orthopaedic Hospital/UCLA School of Medicine, Los Angeles, CA
› Author Affiliations for the THROMBO Research Investigators This work was supported in part by Grant Nos. HL-30616, HL-48259, and HL-07152 from the National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD and by support provided by American Bioproducts Co., Parsippany, NJ.
Further Information

Publication History

Received 24 June 1999

Accepted after revision 15 July 1999

Publication Date:
09 December 2017 (online)

Also available at
    Permissions and Reprints

Summary

Our previous prospective study of post-infarction patients described a strong and significant association of increased plasma D-dimer concentrations in those who experienced a subsequent coronary death or non-fatal myocardial infarction. In the present study, we compare results on stored plasma obtained two months after the index myocardial infarction from 1,038 patients of this trial, using a simple automated latex agglutination (LA) assay in parallel with the standard ELISA test. Results show a somewhat higher mean value for the LA assay (702 ± 1092 vs. 638 ± 986 ng/ml, p = 0.0002), a strong linear correlation of the two assays (r = 0.86) and 88% agreement for values below 500 ng/ml by the ELISA test. D-dimer concentrations determined by each assay were highly correlated in patients with subsequent coronary artery events (p = 0.93) and quartile values for both the LA and ELISA were equally predictive of such events (p = 0.003 and p = 0.001, respectively). This is the first demonstration that a latex agglutination assay for D-dimer can be used to assess the prognostic risk of recurrent coronary thrombotic disease after myocardial infarction

 

  • References

  • 1 Kopec M, Teisseyre E, Dudek-Wojciechowsaa G. Studies on “Double D” fragment from stabilized bovine fibrin. Thromb Res 1973; 2: 283-91.
    CrossrefPubMedGoogle Scholar
  • 2 Gaffney PJ, Brasher M. Subunit structure of the plasmin-induced degradation products of cross-linked fibrin. Biochim Biophys Acta 1973; 295: 308-13.
    CrossrefPubMedGoogle Scholar
  • 3 Pizzo SV, Taylor Jr LM, Schwartz ML, Hill RL, McKee PA. Subunit structure of Fragment D from fibrinogen and cross-linked fibrin. J Biol Chem 1973; 248: 4584-90.
    PubMedGoogle Scholar
  • 4 Bounameaux H, Schneider PA, Slosman D, deMoerloose P, Reber G. Plasma D-dimer in suspected pulmonary embolism: a comparison with pulmonary angiography and ventilation-perfusion scientigraphy. Blood Coagul Fibrinolysis 1990; 1: 577-9.
    PubMedGoogle Scholar
  • 5 Goldhaber SZ, Simons GR, Elliott CG, Haire WD, Toltzis R, Blacklow SC, Doolittle MH, Weinberg DS. Quantitative plasma D-dimer levels among patients undergoing pulmonary angiography for suspected pulmonary embolism. JAMA 1993; 270: 2819-22.
    CrossrefPubMedGoogle Scholar
  • 6 Ginsberg JS, Brill-Edwards PA, Demers C, Donovan D, Panju A. D-dimer in patients with clinically suspected pulmonary embolism. Chest 1993; 104: 1679-84.
    CrossrefPubMedGoogle Scholar
  • 7 Bounameaux H, DeMoerloose P, Perrier A, Reber G. Plasma measurement of D-dimer as diagnostic aid in suspected venous thromboembolism. An overview. Thromb Haemost 1994; 71: 1-6.
    PubMedGoogle Scholar
  • 8 Moss AJ, Goldstein RE, Marder VJ, Sparks CE, Oakes D, Greenberg H, Weiss HJ, Zareba W, Brown MW, Liang C-S, Lichstein E, Little WC, Gillespie JA, Van Voorhees L, Krone RJ, Bodenheimer MM, Hockman J, Dwyer Jr EM, Arora R, Marcus FI, Miller Watelet LF, Case RB. Thrombogenic factors and recurrent coronary events. Circulation 1999; 99: 2517-22.
    CrossrefPubMedGoogle Scholar
  • 9 van Beek EJR, van den Ende B, Berckmans RJ, van der Heide YT, Brandjes DPM, Sturk A, ten Cate JW. A comparative analysis of D-dimer assays in patients with clinically suspected pulmonary embolism. Thromb Haemost 1993; 70: 408-13.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 10 Charles LA, Edwards T, Macik BG. Evaluation of sensitivity and specificity of six D-dimer latex assays. Arch Pathol Lab Med 1994; 118: 1102-5.
    PubMedGoogle Scholar
  • 11 Ginsberg JS, Wells PS, Brill-Edwards P, Donovan D, Panju A, van Beek EJR, Patel A. Application of a novel and rapid whole blood assay for D-dimer in patients with clinically suspected pulmonary embolism. Thromb Haemost 1995; 73: 35-8.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 12 Elias A, Aptel I, Huc B, Chalé JJ, Nguyen F, Cambus JP, Boccalon H, Boneu B. D-dimer test and diagnosis of deep vein thrombosis. A comparative study of 7 assays. Thromb Haemost 1996; 76: 518-22.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 13 Meyer G, Fischer AM, Collignon MA, Benazzouz A, Monge F, Sors H, deRaucourt E. Diagnostic value of two rapid and individual D-dimer assays in patients with clinically suspected pulmonary embolism: comparison with microplate enzyme-linked immunosorbent. Blood Coagul Fibrinolysis 1998; 7: 603-8.
    PubMedGoogle Scholar
  • 14 Escoffre-Barbe M, Oger E, Leroyer C, Grimauz M, Le Moigne E, Nonent M, Bressollette L, Abgrall JP, Soria C, Amiral III JP, Clavier J, Mottier D. Evaluation of a new rapid D-dimer assaya for clinically suspected deep venous thrombosis (Liatest D-dimer). Am J Clin Pathol 1998; 109: 748-53.
    CrossrefPubMedGoogle Scholar
  • 15 Duet M, Benelhadj S, Kedra W, Vilain D, Ajzenberg C, Elkharrat D, Drouet L, Soria C, Mundler O. A new quantitative D-dimer assay appropriate in emergency: reliability of the assay for pulmonary embolism exclusion diagnosis. Thromb Res 1998; 91: 1-5.
    CrossrefPubMedGoogle Scholar
  • 16 Legnani C, Pancani C, Palareti G, Guazzaloca G, Coccheri S. Contribution of a new rapid, quantitative and automated method for D-dimer measurement to exclude deep vein thrombosis in symptomatic outpatients. Blood Coagul Fibrinolysis 1999; 10: 69-74.
    CrossrefPubMedGoogle Scholar
  • 17 Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986; 1: 307-10.
    CrossrefPubMedGoogle Scholar
  • 18 Ginsberg JS, Wells PS, Kearon C, Anderson D, Crowther M, Weitz JI, Bormanis J, Brill-Edwards P, Turpie AG, MacKinnon B, Gent M, Hirsh J. Sensitivity and specificity of a rapid whole-blood assay for D-dimer in the diagnosis of pulmonary embolism. Ann Intern Med 1998; 129: 1006-11.
    CrossrefPubMedGoogle Scholar
  • 19 Janssen MC, Wollersheim H, Verbruggen B, Novakova IR. Rapid D-dimer assays to exclude deep venous thrombosis and pulmonary embolism: current status and new developments. Sem Thromb Hemost 1998; 24: 393-400.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 20 Bernardi E, Prandoni P, Lensing AW, Agnelli G, Guazzaloca G, Scannapieco G, Piovella F, Verlate F, Tomasi C, Moia M, Scarano L, Girolami A. D-dimer testing as an adjunct to ultrasonography in patients with clinically suspected deep vein thrombosis: prospective cohort study. The Multicentre Italian D-dimer Ultrasound Study Investigators Group. BMJ 1998; 17: 1037-40.
    PubMedGoogle Scholar
  • 21 Perrier A, Desmarais S, Miron M-J, deMoerloose P, Lepage R, Slosman D, Didier D, Unger P-F, Patenaude J-V, Bounameaux H. Non-invasive diagnosis of venous thromboembolism in outpatients. Lancet 1999; 353: 190-5.
    CrossrefPubMedGoogle Scholar
  • 22 Kearon C, Julian JA, Newman TE, Ginsberg JS. Noninvasive diagnosis of deep venous thrombosis. McMaster diagnostic imaging practice guidelines initiative. Ann Intern Med 1998; 128: 663-77.
    CrossrefPubMedGoogle Scholar
  • 23 deMoerloose P, Michiels JJ, Bounameaux H. The place of D-dimer testing in an integrated approach of patients suspected of pulmonary embolism. Sem Thromb Hemost 1998; 24: 409-12.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 24 Bloom BJ, Tucker LB, Miller LC, Schaller JG. Fibrin D-dimer as a marker of disease activity in systemic onset juvenile rheumatoid arthritis. J Rheumatol 1998; 25: 1620-5.
    PubMedGoogle Scholar
  • 25 Oya M, Akiyama Y, Yanagida T, Akao S, Ishikawa H. Plasma D-dimer level in patients with colorectal cancer: its role as a tumor marker. Surg Today 1998; 28: 373-8.
    CrossrefPubMedGoogle Scholar
  • 26 Kollef MH, Eisenberg PR, Shannon W. A rapid assay for the detection of circulating D-dimer is associated with clinical outcomes among critically ill patients. Crit Care Med 1998; 26: 1054-60.
    CrossrefPubMedGoogle Scholar
  • 27 Wada H, Sakuragawa N, Shiku H. Hemostatic molecular markers before onset of disseminated intravascular coagulation in leukemic patients. Sem Thromb Hemost 1998; 24: 293-7.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 28 Murtha AP, Boggess KA, Jimmerson CE, Greig PC, Herbert WN. Umbilical venous D-dimer concentrations with and without labor. Obstet Gynecol 1998; 92: 184-6.
    PubMedGoogle Scholar
  • 29 Cushman M, Lemaitre RN, Kuller LH, Psaty BM, Macy EM, Sharrett AR, Tracy RP. Fibrinolytic activation markers predict myocardial infarction in the elderly. The Cardiovascular Health Study. Arterioscler Thromb Vasc Biol 1999; 19: 493-8.
    CrossrefPubMedGoogle Scholar