Stimulation of Proteinase Activated Receptor-2 Causes Endothelial Cells to Promote Blood Coagulation In Vitro

Anna-Karin Alm; Eva Norström; Johan Sundelin; Sverker Nystedt

doi:10.1055/s-0037-1614610

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1999; 81(06): 984-988
DOI: 10.1055/s-0037-1614610

Letters to the Editor

Schattauer GmbH

Stimulation of Proteinase Activated Receptor-2 Causes Endothelial Cells to Promote Blood Coagulation In Vitro

Anna-Karin Alm

¹From the Division of Molecular Neurobiology, Department of Physiology and Neuroscience, Lund University, Sweden

,

Eva Norström

¹From the Division of Molecular Neurobiology, Department of Physiology and Neuroscience, Lund University, Sweden

,

Johan Sundelin

¹From the Division of Molecular Neurobiology, Department of Physiology and Neuroscience, Lund University, Sweden

,

Sverker Nystedt

¹From the Division of Molecular Neurobiology, Department of Physiology and Neuroscience, Lund University, Sweden

› Author Affiliations

Abstract

Summary

Proteolytically activated receptors define a new subclass among the G-protein coupled receptors. Proteinase activated receptor-2 (PAR-2), the second member to be identified of this growing receptor subclass, can be activated by trypsin and trypsin-like serine proteases such as mast cell tryptase. PAR-2 is expressed in endothelial cells. Here we have studied if activation of PAR-2 changes the coagulation properties of cultured human umbilical vein endothelial cells. We show that activation of PAR-2 induces rapid and transient formation of tissue factor mRNA with a maximum level 1 hour after receptor stimulation. The increased mRNA level was accompanied by an increased tissue factor activity at the endothelial cell surface, shortening coagulation time in a standard clotting assay. The level of tissue factor activity after PAR-2 activation was comparable with the effects of thrombin receptor (PAR-1) activation although neither of the two protease receptors were as strong inducers of tissue factor as tumor necrosis factor-α.

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References

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