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Thromb Haemost 1999; 81(06): 984-988
DOI: 10.1055/s-0037-1614610
Letters to the Editor
Schattauer GmbH

Stimulation of Proteinase Activated Receptor-2 Causes Endothelial Cells to Promote Blood Coagulation In Vitro

Anna-Karin Alm
1   From the Division of Molecular Neurobiology, Department of Physiology and Neuroscience, Lund University, Sweden
,
Eva Norström
1   From the Division of Molecular Neurobiology, Department of Physiology and Neuroscience, Lund University, Sweden
,
Johan Sundelin
1   From the Division of Molecular Neurobiology, Department of Physiology and Neuroscience, Lund University, Sweden
,
Sverker Nystedt
1   From the Division of Molecular Neurobiology, Department of Physiology and Neuroscience, Lund University, Sweden
› Author Affiliations
Further Information

Publication History

Received 07 December 1998

Accepted after revision 23 February 1999

Publication Date:
09 December 2017 (online)

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Summary

Proteolytically activated receptors define a new subclass among the G-protein coupled receptors. Proteinase activated receptor-2 (PAR-2), the second member to be identified of this growing receptor subclass, can be activated by trypsin and trypsin-like serine proteases such as mast cell tryptase. PAR-2 is expressed in endothelial cells. Here we have studied if activation of PAR-2 changes the coagulation properties of cultured human umbilical vein endothelial cells. We show that activation of PAR-2 induces rapid and transient formation of tissue factor mRNA with a maximum level 1 hour after receptor stimulation. The increased mRNA level was accompanied by an increased tissue factor activity at the endothelial cell surface, shortening coagulation time in a standard clotting assay. The level of tissue factor activity after PAR-2 activation was comparable with the effects of thrombin receptor (PAR-1) activation although neither of the two protease receptors were as strong inducers of tissue factor as tumor necrosis factor-α.

 

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