Abstract
Structure elucidation using NMR spectroscopy has become a vital part of the toolkit
of modern synthetic chemistry. Characterisation of final products, quality control
of production, analysis of complex mixtures in synthetic method development, and structure
elucidation of isolated natural products are examples where NMR spectroscopy is a
part of daily routine. The two factors that usually limit the applicability of NMR
are resolution and sensitivity. The experimental method described in this Account,
real-time pure shift acquisition, yields heteronuclear correlation spectra such as
HSQC that offer significant improvements in both resolution and sensitivity, at negligible
cost to the analyst. The advantages that real-time pure shift acquisition enjoys over
conventional experiments are discussed and illustrated with selected examples including
carbohydrate and alkaloid mixtures. Advanced data acquisition and processing techniques
that reduce experiment time and are easily combined with pure shift NMR methods are
also described.
1 Introduction
2 Simultaneous Sensitivity and Resolution Enhancement Using Real-Time Acquisition
in HSQC
3 Processing Pure Shift Data
4 Pulse Sequences for Real-Time Pure Shift HSQC
5 Conclusions and Future Perspectives
Key words
NMR - HSQC - pure shift - homodecoupling - BIRD - NUS - linear prediction
