J Knee Surg 2018; 31(07): 664-669
DOI: 10.1055/s-0037-1606549
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Comparative Analysis of Gene Expression between Cartilage and Menisci in Early-Phase Osteoarthritis of the Knee—An Animal Model Study

Jun Endo
1  Department of Orthopaedic Surgery, Chiba University, Graduate School of Medicine, Chiba, Japan
,
Takahisa Sasho
1  Department of Orthopaedic Surgery, Chiba University, Graduate School of Medicine, Chiba, Japan
2  Center for Preventive Medical Sciences, Chiba University, Cyuou Chiba, Chiba, Japan
,
Ryuichiro Akagi
1  Department of Orthopaedic Surgery, Chiba University, Graduate School of Medicine, Chiba, Japan
,
Yuta Muramatsu
1  Department of Orthopaedic Surgery, Chiba University, Graduate School of Medicine, Chiba, Japan
,
Atsuya Watanabe
3  Department of General Medical Services, Chiba University, Graduate School of Medicine, Chiba, Japan
,
Yorikazu Akatsu
1  Department of Orthopaedic Surgery, Chiba University, Graduate School of Medicine, Chiba, Japan
,
Taisuke Fukawa
1  Department of Orthopaedic Surgery, Chiba University, Graduate School of Medicine, Chiba, Japan
,
Masamichi Tahara
4  Department of Orthopaedic Surgery, Eastern Chiba Medical Center, Chiba, Japan
,
Satoshi Yamaguchi
1  Department of Orthopaedic Surgery, Chiba University, Graduate School of Medicine, Chiba, Japan
› Author Affiliations
Further Information

Publication History

03 April 2017

04 August 2017

Publication Date:
15 September 2017 (eFirst)

Abstract

Cartilage degeneration is believed to be the primary event in the development of osteoarthritis (OA). On the other hand, meniscal degeneration is observed with high prevalence, and some researchers have pointed out that pathological changes in menisci precede that of cartilage. The purpose of the present study is to investigate comprehensive gene expression pattern of cartilage and menisci in the initial phase of surgically induced OA and to compare them. Secondary OA was surgically induced in 10-week-old male Wistar rats by anterior cruciate ligament transection (ACLT). Articular cartilage and menisci were separately dissected from six ACLT- and six sham-operated rats. Each specimen was analyzed by microarray, histological, and immunohistochemical analysis 3 weeks after surgery. Of the 36,685 transcripts detectable by microarray, the number of upregulated transcripts in ACLT menisci was >2.5-fold compared with that in ACLT menisci in any given threshold. Cluster analysis using the Database for Annotation Visualization and Integrated Discovery (DAVID) showed genes related to OA, such as response to stimulus, angiogenesis, and apoptosis, which were predominantly found in menisci in ACLT rats. Representative proteases including Adamts2, 4, Mmp2, 12, 13, 14, 16, extracellular matrix genes including versican (Vcan), lumican (Lum), syndecan1 (Sdc1), and Prostaglandin endoperoxide synthase2 (Ptgs2) were up-regulated in menisci, but were not up-regulated in cartilage. Our results indicated that the molecular changes that occurred in menisci preceded those occurred in cartilage in the very early phase of surgically induced OA models.