Am J Perinatol 2017; 34(09): 911-917
DOI: 10.1055/s-0037-1601311
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

sRAGE Is Elevated in the Lungs of Premature Infants Receiving Mechanical Ventilation

Henry J. Rozycki
1  Division of Neonatal Medicine, Children's Hospital of Richmond at VCU, Richmond, Virginia
Jennifer Bradley
2  Department of Pediatrics, Children's Hospital of Richmond at VCU, Richmond, Virginia
Simon Karam
3  Division of Neonatal Medicine, Department of Pediatrics, University of Mississippi, Jackson, Mississippi
› Author Affiliations
Further Information

Publication History

10 November 2016

16 February 2017

Publication Date:
20 March 2017 (online)


Background Soluble receptor for advanced glycation end-products (sRAGE), a soluble isoform of the RAGE receptor, is elevated in lungs from patients with acute conditions such as acute respiratory distress syndrome and bronchiolitis. This study investigated whether sRAGE is present in ventilated infants.

Methods Tracheal aspirates from the first week or the fifth week of life were obtained from intubated very low birth weight subjects and analyzed by Western blot. Immunohistochemistry analysis for sRAGE was performed on paraffin-embedded lung autopsy slides from 19 other infants.

Results The sRAGE band densities were similar among the seven infants who fully recovered, eight who developed bronchopulmonary dysplasia (BPD), and 5 who died (analysis of variance p = 0.797) but was higher at 4 weeks, p = 0.0324. There was minimal sRAGE staining in the autopsied lungs from previable infants (20–21 weeks) or from those who were not ventilated or had mild lung disease. In contrast, substantial staining was present in two of three with BPD, and those who received high ventilatory support.

Conclusion sRAGE is present in ventilated infants. Levels are generally higher in those who receive prolonged or vigorous mechanical ventilation. Since sRAGE may have roles in inflammation and cell adherence, its role in the development of BPD may warrant study.


A portion of this work was presented at the ATS 2016 International Conference, San Francisco, CA, May 15, 2016.