Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1597042
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

The neuroprotective activity of a proanthocyanidin enriched Ginkgo biloba L. leaves extract in vitro and in vivo

K Kuchta
1   National Institute of Health Sciences, Division of Pharmacognosy, Phytochemistry and Narcotics, Tokyo, Japan
,
HX Qiao
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
,
HB Huang
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
,
L Fang
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
,
Y Chen
4   Zhejiang University of Technology, Hangzhou, China
,
RW Wang
2   Zhejiang CONBA Pharmaceutical, Hangzhou, China
3   Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology, Hangzhou, China
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

The commercial refined extract of Ginkgo biloba L. leaves can protect brain tissue against cerebral ischemia/reperfusion (I/R) injury [1], an activity attributed to the antioxidant activity of its flavonoids and proanthocyanidins. The latter have not been sufficiently studied up to now. Thus, this study aims to evaluate the neuroprotective effects of a proanthocyanidin enriched G. biloba leaves extract (GPE) – > 90% proanthocyanidins after resin HPD100C adsorption – on cerebral I/R injury compared to commercial proanthocyanidins from grape seeds (Vitis vinifera L.) (GSP) and nimodipine as positive control. In vitro, the tissue protective effect of GPE was measured using PC12 cells, cultured in 96-well plates. After addition of GEP or GSP (0.625, 1.25, 2.50 µg/ml) and 12h of cultivation, cells were incubated with H2O2 (40mM) for another 24h. Cell damage was assayed using a commercial LDH assay kit. GPE markedly ameliorated the increase in LDH release and thus the respective decrease in cell viability. For the in vivo assay, male Sprague-Dawley rats were divided into 6 groups (sham: 8, placebo: 25, GPE 80 mg/kg: 13, GPE 40 mg/kg: 13, GPE 20 mg/kg: 16, GSE 40 mg/kg: 18, nimodipine: 8). All non-sham animals were subjected to cerebral I/R injury by occluding the middle cerebral artery with a nylon suture that was removed after 2h of ischemia to establish reperfusion. After recovery from anaesthesia, the rats were returned to their cages with free access to water and food. All animals were sacrificed 24h after reperfusion. Coronal brain sections were stained and the infarct ratio calculated. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels in the brain tissue were measured using commercial assay kits. Under treatment of cerebral I/R injury in test animals with GPE, the death rate decreased, neurological dysfunctions were ameliorated, and both average infarct size and concentrations of MDA and SOD were significantly reduced as compared to the placebo group.

Keywords: Ginkgo biloba L., proanthocyanidins, neuroprotective activity, cerebral ischemia, reperfusion injury.

References:

[1] Hu B, Sun S, Mei G, Chen L, Tong E. Protective effects of Ginkgo biloba extract on rats during cerebral ischemia/reperfusion. Chin Med J (Engl) 2002; 115: 1316 – 1320