Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596916
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Comparison of antioxidant and enzyme inhibition activities as well chemical composition of different extracts and fractions of Rubus caesius leaves

W Skowronska
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Warsaw Medical University, ul. Banacha 1, 02 – 097 Warsaw, Poland
,
DM Grochowski
2   Department of Pharmacognosy, Medical University of Bialystok, ul. Mickiewicza 2a, 15 – 230 Białystok, Poland
,
A Bazylko
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Warsaw Medical University, ul. Banacha 1, 02 – 097 Warsaw, Poland
,
S Granica
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Warsaw Medical University, ul. Banacha 1, 02 – 097 Warsaw, Poland
,
M Tomczyk
2   Department of Pharmacognosy, Medical University of Bialystok, ul. Mickiewicza 2a, 15 – 230 Białystok, Poland
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Publikationsverlauf

Publikationsdatum:
14. Dezember 2016 (online)

 

The aim of our study was determination of antioxidant and anti-inflammatory activities, and chemical composition of extracts and fractions (water RC1, 50% methanol RC2, methanol RC3, diethyl ether RC4, ethyl acetate RC5 and n-butanol RC6) obtained solvents with different polarity from Rubus caesius leaves [1]. Scavenging of DPPH radicals, as well superoxide anion (O2 -·) scavenging activity in xanthine-xanthine oxidase system of the tested extracts and fractions were evaluated. To examine the enzyme inhibitory activity of the studied extracts and fractions, inhibition of lipoxidase and xanthine oxidase activity was tested. The range of the extracts and fractions concentration was: 10 – 150, 10 – 100, 50 – 200 and 10 – 100 µg/mL for DPPH, O2 -·, lipoxidase and xanthine oxidase, respectively. All mentioned assays were made in in vitro cell-free systems [2,3]. The most active against DPPH radicals was the RC4 fraction with SC50 about 11 µg/mL. While the strongest scavenging activity against O2 -· showed the RC2 extract with observed dose dependent activity (SC50 about 17 µg/mL). Enzymes inhibition activity of the tested extracts was rather weak. Studies of composition of the most active extracts and fractions were performed using HPLC coupled with DAD and Ion Trap Mass Detector. HPLC analyses of samples were carried out on a reversed-phase Zorbax SB-C18, 150 × 2.1 mm, 1.9 µm column (Agilent, Palo Alto, California, USA). The mobile phase (A) was water/formic acid (100:0.1, v/v) and the mobile phase (B) was acetonitrile/formic acid (100:0.1, v/v). A two-step gradient system was used: 0 – 50 min. 1 – 26% B and 50 – 60 min 26 – 95% B. The flow rate was 0.300 mL/min. In the hydromethanolic extract (RC2) ellagic acid and sanguiniin H-6 were detected as the dominating compounds. The RC4 fraction contained a mixture of phenolic acids, flavonoids as well as ellagitannins and related compounds.

Keywords: Rubus caesius, DPPH, xanthine oxidase, lipoxidase, HPLC-DAD-MS

References:

[1] Rejewska A, Sikora A, Tomczykowa M, Tomczyk M. Rubus caesius. Phcog Comm 2013, 3: 55 – 57.

[2] Bazylko A, Piwowarski JP, Filipek A, Bonarewicz Jessica, Tomczyk M. In vitro antioxidant and anti-inflammatory activities of extracts from Potentilla recta and its main ellagitannin, agrimoniin. J Ethnopharmacol 2013, 149: 222 – 227.

[3] Bazylko A, Granica S, Filipek A, Piwowarski J, Stefańska J, Osińska E, Kiss AK. Comparison of antioxidant, anti-inflammatory, antimicrobial activity and chemical composition of aqueous and hydroethanolic extracts of the herb of Tropaeolum majus L. Ind Crop Prod 2013, 50: 88 – 94.