Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596531
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Chemical composition and bioactivity of the essential oil of Pinus roxburghii bark

RM Labib
1   Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, 11566, Cairo, Egypt
2   National Center for Natural Products Research, University of Mississippi, University, Mississippi, 38677, US
,
FS Youssef
1   Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, 11566, Cairo, Egypt
,
ML Ashour
1   Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, 11566, Cairo, Egypt
,
J Búfalo
3   Institute of Biosciences (IB), UNESP-Univ., Estadual, Paulista, Botucatu, Sao Paulo, Brazil
4   United States Department of Agriculture, Agricultural research Service, Natural Products Utilization Research Unit, University of Mississippi, University, MS, 38677, USA
,
SA Ross
2   National Center for Natural Products Research, University of Mississippi, University, Mississippi, 38677, US
5   Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, 38677, USA
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Essential oils are volatile secondary metabolites that are normally produced and can be used safely to treat several health disorders [1]. The essential oil obtained from Pinus roxburghii Sarg. bark (Pinaceae) cultivated in Egypt was qualitatively and quantitatively analyzed using GC/FID and GC/MS techniques [2]. Seventy five components representing 89.65% of the total hydrodistilled oil were identified, with longifolene (33.13%) representing the major component. The sequiterpene hydrocarbons and the oxygenated sequiterpenes constitute 37.13 and 19.79% respectively. The essential oil was assessed for its antimicrobial activity against two Gram positive, two Gram negative, methicillin resistant Staphylococcus aureus (MRSA) and five fungal strains. It exhibited mild antimicrobial activity showing IC50 values exceeding 20 µg/mL. Moreover, in vitro antimalarial activity was investigated against both chloroquine sensitive (D6) and resistant (W2) strains of Plasmodium falciparum in which the oil exerted a mild activity. The antileishmanial activity was evaluated in vitro against different cultures of Leishmania donovani promastigotes and amastigotes. It was also tested against Trypanosoma brucei, the oil showed a mild antileishmanial and trypanocidal activities, with IC50 values exceeding 10 µg/mL. Concerning its anti-inflammatory activity, no inhibitory effect of the essential oil on the cannabinoids or opioids receptors was shown. In silico molecular docking of its major components was performed on human glucocorticoids receptor (GR). Results clarified that longifolene, possess an affinity to GR binding site comparable to the potent anti-inflammatory GR agonist, dexamethasone. The binding energy showed that longifolene has the higher binding affinity (-28.76 kcal/mol) revealing a significant anti-inflammatory activity as compared to the other oil components.

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Acknowledgements: We are thankful to Dr. Babu Tekwani, Dr. Shabana Khan and Dr. Melissa Jacob, National Center for Natural Products Research, The University of Mississippi, School of Pharmacy, Mississippi, USA for the antileishmanial, antimalarial and antimicrobial assays.

Keywords: Pinus roxburghii, sssential oil, GC/MS, anti-infective, anti-inflammatory, molecular docking.

References:

[1] Husnu Can Baser K, Buchbauer G. Handbook of essential oils, science, technology and applications. CRC press, 2010

[2] Adams RP. Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. Carol Stream, IL: Allured Pub Corp, 2004

[3] El-Readi MZ, Eid HH, Ashour ML, Eid SY, Labib RM, Sporer F, Wink M. Variations of the Chemical Composition and Bioactivity of Essential Oils from Leaves and Stems of Liquidambar styraciflua (Altingiaceae). J Pharm Pharmacol 2013; 65: 1653 – 1663