Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596487
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Simultaneous quantitation of five bioactive components of Menthae Herba by HPLC/PDA

MH Woo
1   College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Republic of Korea
,
BT Zhao
1   College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Republic of Korea
,
MJ Choi
1   College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Republic of Korea
,
YH Kim
2   College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea
,
JS Kang
2   College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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Several different methods have been developed to qualify and quantitate the components in Menthae Herba by HPLC [1 – 5]. However, each of these techniques possesses regretful weakness that the HPLC method was untested on the acidic effect for the mobile phase. Therefore the peaks of diosmin and rosmarinic acid which were completely overlapped have not been separated. Hence, we developed a new method for simultaneous determination of five marker compounds (hesperidin, rosmarinic acid, diosmin, didymin, and buddleoside) in Menthae Herba by HPLC/PDA. Five marker components were separated with a Phenomenex Gemini C18 (250 mm × 4.6 mm, 5 µm) column by one step gradient elution using 0.3% trifluoroacetic acid in methanol (A) and water (B) as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 285nm. The new developed method was successfully validated and used in the analysis of Menthae Herba. Validation results were satisfactory for linearity (r 2> 0.999), recovery (101.9˜108.7%), precision (< 0.21%), accuracy (100.50˜111.00%), stability (98.47˜101.01%) and robustness. In conclusion, the results indicated that the established HPLC/PDA method is suitable for quantitation for quality evaluation of Menthae Herba.

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Acknowledgements: This work was supported by a grant from the National Center for Standardization of Herbal Medicine funded by the Ministry of Food and Drug Safety, Republic of Korea (15172MFDS189).

Keywords: Menthae Herba, HPLC, drying condition, rosmarinic acid.

References:

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