Chemical constituents from Agrimonia pilosa with their protein tyrosine phosphatase and acetylcholinesterase inhibitory activities

Agrimonia pilosa Ledeb., a perennial herb, belongs to the Rosaceae family. The whole plant A. pilosa has been used traditionally as hemostatic, antimalarial, and antidysenteric in Chinese herbal medicine for a long time [1,2]. Two new flavanonol glucoside isomers, (2S,3R) dihydrokaempferol 3-O-β-<scaps>D</scaps>-glucoside (1) and (2R,3S) dihydrokaempferol 3-O-β-<scaps>D</scaps>-glucoside (2), were isolated from the aerial parts of A. pilosa, along with 12 known compounds (3-14). Their structures were determined on the basis of spectroscopic analyses. In addition, all the isolates were evaluated for PTP1B inhibitory activity. Among them, compounds 13 and 14 displayed potential inhibitory activity against PTP1B with IC₅₀ values of 7.14 ± 1.75 and 7.73 ± 0.24µM, respectively. Furthermore, all the isolated compounds were found to inhibit AChE with IC₅₀ values ranging from 62.96 ± 0.35 to 118.32 ± 0.09µM.

Keywords: Agrimonia pilosa, Flavanonol glucosides, Protein tyrosine phosphatase 1B, acetylcholinesterase.

References:

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