Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596364
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

In vitro antioxidant and anticancer properties of hydroethanolic extracts, fractions and novel flavones of Ageratum conyzoides leaves

F Acheampong
1   Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi-Ghana
2   South Dakota State University, Brookings, SD 57007, USA
,
C Larbie
1   Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi-Ghana
,
R Appiah-Oppong
3   Department of Clinical Pathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon-Ghana
,
J Reilly
4   Novartis Institutes for BioMedical Research, Cambridge, MA 02139, USA
,
M Spencer
4   Novartis Institutes for BioMedical Research, Cambridge, MA 02139, USA
,
FKN Arthur
1   Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi-Ghana
,
I Tuffour
3   Department of Clinical Pathology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon-Ghana
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Ageratum conyzoides L (Asteraceae) has increasingly been used in folklore for the treatment of a wide range of diseases including cancer [1] This study was aimed at investigating the anticancer potential of 50% hydroethanolic leaf extracts, fractions and compounds isolated from Ageratum conyzoides against Jurkat cells using standard methods This study also reports the isolation of a novel flavone, 8-chloro-5-hydroxy-2-(4-hydroxy-3,5-dimethoxyphenyl)-7-methoxy-4 H-chromen-4-one Mean IC50 values were compared by one way ANOVA The 50% hydroethanolic leaf extract as well as the chloroform and ethylacetate fractions exhibited cytotoxicities against Jurkat cells with IC50 values of 151 ± 03 µg/ml, 636 ± 16 µg/ml and 46 ± 01 µg/ml, respectively compared to curcumin, 0002 ± 00 µg/ml and had strong selectivity indices (SI) against Jurkat cells with values of 6633, 1572 and 2174, respectively compared to curcumin (SI = 23) Treatments with purified flavones (5,6,7,3',4',5'-Hexamethoxyflavone, 5,6,7,8,3',4'-Hexamethoxyflavone and 5,6,7,3',4'-Pentamethoxyflavone) showed strong cytotoxic effect in a dose responsive manner Only 5,6,7,3',4',5'-Hexamethoxyflavone and 5,6,7,8,3',4'-Hexamethoxyflavone induced apoptosis through caspase 3/7 activation in a dose-dependent manner compared to staurosporine, positive control For the first time 5,6,7,3',4',5'-Hexamethoxyflavone and 5,6,7,8,3',4'-Hexamethoxyflavone have been shown to exert cytotoxic effects against Jurkat cells via induction of apoptosis Physicochemical properties such as albumin binding (% HSA), affinity to phosphatidylcholine (CHI IAM74 scores) and volume of distribution (Log Vd) predicted for these compounds assume that the cytotoxicity observed is due to on-target action and not off-target effects This suggests that these bioactive components found in A conyzoides could be useful in the development of new leukemic therapies

Keywords: Ageratum conyzoides, cytotoxicity, flavone, Jurkat.

References:

[1] Acheampong F, Larbie C, Appiah-Opong R, Arthur FKN, Tuffour I. In vitro antioxidant and anticancer properties of hydroethanolic extracts and fractions of Ageratum cornyzoides. EJMP 2015; 7: 204 – 215