Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596352
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Neuroprotective compounds from Euonymus alatus

YS Jung
1   Department of Medical Biomaterials Engineering, College of Biomedical science, Kangwon National University, Chuncheon 200 – 701, Korea
,
JB Weon
1   Department of Medical Biomaterials Engineering, College of Biomedical science, Kangwon National University, Chuncheon 200 – 701, Korea
,
CJ Ma
1   Department of Medical Biomaterials Engineering, College of Biomedical science, Kangwon National University, Chuncheon 200 – 701, Korea
2   Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200 – 701, Korea
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Glutamate is presented as a neurotransmitter in the central nervous system [1]. However, the overexpression of glutamate leads to several neurodegenerative disorders such as Alzheimer's disease [2,3]. The n-hexane fraction of Euonymus alatus was shown to have a neuroprotective effect against glutamate-induced oxidative toxicity in HT22 cells. In this study, moretenone (1), moretenol (2), friedelanol (3), lupenone (4), β-sitosterol (5), betulin (6), undecanoic acid, 1,2-phenylene ester (7), glycerol 1-tetracosanoate (8), methyl hydrogen tetradecanedioate (9) and 10,13-Nonadecadienoic acid, methyl ester (10) were isolated from n-hexane fraction of E. alatus using the open column chromatography (silica gel, Sephadex LH-20), medium pressure liquid chromatography (MPLC) and preparative high-performance liquid chromatography (prep-HPLC). Chemical structures of all compounds were established using 1H-NMR, 13C-NMR and Mass spectrum. Their neuroprotective activity was evaluated by MTT assay. Betulin (6) and methyl hydrogen tetradecanedioate (9) exhibited a neuroprotective activity. Betulin (6) and methyl hydrogen tetradecanedioate (9) decreased ROS level and intracellular Ca2+ level. In addition, betulin (6) and methyl hydrogen tetradecanedioate (9) increased MMP, total glutathione level, glutathione reductase activity, glutathione peroxides activity. These results suggested that neuroprotective ability of the betulin (6) and methyl hydrogen tetradecanedioate (9) is associated with antioxidant activity.

Acknowledgements: This research was supported by Kangwon National University.

Keywords: Euonymus alatus, neuroprotective effect, oxidative stress, isolation of compounds.

References:

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