Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596323
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Effects of extracts from leaves and flowers from Syringa vulgaris on mediators of inflammation in a human neutrophils model

M Woźniak
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1 st., 02 – 097 Warsaw, Poland
,
K Psyk
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1 st., 02 – 097 Warsaw, Poland
,
M Biernat
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1 st., 02 – 097 Warsaw, Poland
,
ME Czerwińska
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1 st., 02 – 097 Warsaw, Poland
,
AK Kiss
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1 st., 02 – 097 Warsaw, Poland
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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Syringa vulgaris L. (Common lilac) from the Oleaceae family is traditionally used for treating inflammation-associated diseases, such as rheumatoid arthritis, gout, common cold, diabetes mellitus, gastrointestinal disorders and externally to soothe the skin irritation. These diseases are closely associated with an excessive inflammatory response of stimulated neutrophils [1]. The aim of the study was to examine the effect of 60% ethanolic extracts and selected fractions from leaves and flowers for their effect on pro-inflammatory functions of stimulated neutrophils such as: reactive oxygen species (ROS), interleukin 8 (IL-8), tumor necrosis factor alpha (TNFα) production/release. Ethanolic extracts and fractions were characterized by HPLC-DAD-MS/MS. Fractions were obtained using Diaion HP20 column chromatography. The inhibition of ROS production by neutrophils was determined using luminol- dependent chemiluminescence. The effect on IL-8 and TNFα production by neutrophils was measured by an enzyme-linked immunosorbent assay. The potential cytotoxicity of tested extracts/fractions was analyzed with flow cytometry after propidium iodide staining. Analyzed extracts contain caffeic acid derivatives as dominating compounds followed by secoiridoids such as oleuropein and its derivatives. Extracts from both leaves and flowers significantly reduced the f-MLP induced reactive oxygen species production at concentrations of 25 – 100 µg/mL. Extracts were shown to modulate lipopolysaccharide (LPS)-triggered production of IL-8 (at 25 – 100 µg/mL) but no the production of TNF-α. Interestingly, some fractions obtained from primary extracts were able to decrease cytokines productions but others significantly stimulated IL-8 and TNF-α release. Our observations suggested that Common lilac may contain compounds showing immunomodulating activity and could play a role in the treating of inflammatory disease.

Acknowledgements: This study was supported by research grant 2015/17/B/NZ7/03086 from Polish National Science Center.

Keywords: Syringa, common lilac, neutrophils, inflammation.

References:

[1] Amulic B, Cazalet C, Hayes GL, Metzler KD, Zychlinsky A. Neutrophil function: From mechanisms to disease. Ann Rev of Immunol 2012; 30: 459 – 489