Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596280
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Using modern 2D-high performance thin layer chromatography coupled with MALDI-TOF-MS for a first screening approach of plant extracts

M Oberle
1   Merck KGaA, Frankfurter Strasse 250, Darmstadt, Germany
,
K Behrend
2   University Hamburg, Hamburg School of Food Science, Grindelallee 117, Hamburg, Germany
,
P Lewits
1   Merck KGaA, Frankfurter Strasse 250, Darmstadt, Germany
› Institutsangaben
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Publikationsverlauf

Publikationsdatum:
14. Dezember 2016 (online)

 

2D development on modern thin layer chromatography in combination with the mass spectrometric method MALDI-TOF-MS (matrix-assisted laser desorption/ionization time of flight mass spectrometry) combines the advantage of a multi development high performance chromatographic separation with a high resolution mass spectrometric method [1]. The combination of these two powerful methods is very helpful for fast and, nearly complete characterization. Plant extracts are difficult to separate, due to their high number of single compounds [2,3]. Different solvent gradients (stepwise) with different polarities in the two-dimensional chromatography lead to better separation results compared to one-dimensional chromatography and gain various helpful information for the design of following clean-up processes. Nevertheless, not fully separated clusters of substances can be analyzed by MALDI-TOF-MS, which makes it possible to separate substances within the mass spectrometric measurement. An intuitive visualization of the results makes HPTLC- MALDI-MS coupling a useful method for the analysis of complex plant extract raw materials and new products.

The technique of coupling 2D thin layer chromatography with MALDI-TOF-MS is demonstrated by the identification of Flavonoids and other contents in Walteria Indica and Eugenia uniflora extracts.

Keywords: Thin Layer Chromatography, TLC, High Performance Thin Layer Chromatography, HPTLC, matrix-assisted laser desorptionionization time of flight mass spectrometry, TLC-MALDI-MS, TLC-MS coupling techniques, screening of plant extracts.

References:

[1] Fuchs B, Süß R, Nimptsch A, Schiller J. MALDI-TOF-MS directly combined with TLC: A review of the current state. Chromatographia 2009; 69: 95 – 105

[2] González B, Vogel H, Razmilic I, Wolfram E. Polyphenol, anthocyanin and antioxidant content in different parts of maqui fruits (Aristotelia chilensis) during ripening and conservation treatments after harvest. Ind Crops Prod 2015; 76: 158 – 165

[3] Hawryl MA, Waksmundzka-Hajnos M. Micro 2D-TLC of selected plant extracts in screening of their composition and antioxidative properties. Chromatographia 2013; 76: 1347 – 1352