Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596233
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Comprehensive HPLC-DAD based metabolomics analysis of Tanacetum species from Iran

H Rezadoost
1   Departement of phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran
,
R Alizadeh
1   Departement of phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran
,
M Mirzaei
2   Department of Computational Biology, Faculty of High Technologies, Tarbiat Modares University, Tehran, Iran
,
A Sonboli
3   Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran
,
M Goudarzi
4   KU Leuven, Department of Biosystems, MeBioS, Kasteelpark Arenberg 30, 3001 Leuven, Belgium
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Today, metabolomics, combination of chromatographic fingerprinting and high throughput data analysis, which could be based on both known and unknown chemical constituents have proven to give a more reliable and comparable plant chemical profile [1]. The genus Tanacetum L. (Asteraceae-Anthemideae) is represented in Iran by 36 species in which some species e.g., Tanacetum parthenium (L.) Sch.-Bip. has been utilized in traditional medicine for migraine and cancer [2]. In the present study the sesquiterpene lactone content in different Tanacetum species was characterized to evaluate the interspecific differentiation. The powdered plant samples were extracted by acetonitrile-water (9:1) and then analyzed using an optimized SPE-HPLC-DAD. Thereafter, HPLC-DAD data from duplicate of 36 Tanacetum species was exported to in-house scripting in R programming environment and then all data were pre-processed with regard to baseline correction, normalization and alignment. Simultaneous evaluation of all available data using principal component analysis based on chromatographic profiles confirmed the separation of Tanacetum species into three groups. Our finding showed that two deviating Tanacetum species, i.e., T. paradoxum Bornm. and T. fruticulosum Ledeb. is clearly distinct from other Tanacetum species and our data suggests that they should be excluded from the genus.

Acknowledgements: This study was granted by Shahid Beheshti University research council.

Keywords: Tanacetum species, HPLC-DAD, fingerprints analysis.

References:

[1] Goodarzi M, Russell PJ, Vander Heyden Y. Similarity analyses of chromatographic herbal fingerprints: a review. Analyt Chim Acta 2013; 804: 16 – 28

[2] Esmaeili MA, Sonboli A, Noushabadi MA. Antioxidant and protective properties of six Tanacetum species against hydrogen peroxide-induced oxidative stress in K562 cell line: A comparative study. Food Chem 2010; 121: 148 – 155