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NMR and LC-TOF-MS metabolomic analysis reveals inter- and intraspecific variation and osmotic stress response of chemical profile in Perovskia species
14 December 2016 (online)
In this paper, we focus on two Perovskia species, P. abrotanoides Karel. and P. atriplicifolia Benth. (Lamiaceae), used in local folk phytotherapy in Iran, Afghanistan, Pakistan, and other countries in the region. We also included three popular P. atriplicifolia ornamental varieties 'Blue Spire', 'Little Spire', and 'Lacey Blue'. The experimental approach involved pot and hydroponic cultures treated with NaCl and silicon. All samples were prepared according to  and analyzed with NMR and UPLC-HR-MS  for differential screening of terpenoid and phenylpropanoid profiles. Targeted comparison of metabolic profiles of Perovskia sp. revealed significant, previously not reported differences between the two species and the ornamental varieties. In total, four major hydroxycinnammic oligomers and glycosides (three- to pentameric salvianolic and clinopodic acids) and over twenty abietane and icetaxane diterpenoids were present in both species. P. atriplicifolia roots contained significantly higher number of diterpenoids, more than 12 additional different compounds compare with P. abrotanoids, as well as higher total amounts of polyphenols in P. abrotanoides/P.atriplicifolia (1.1% and 1.9%) and diterpenes (1.7% and 3.4%) respectively. Stress treatment (50, 100, 150 mmol NaCl) additionally induced quantitative variation leading to alteration in relative amounts of phenolic acids and tanshinones. Silicone treatment increased tolerance to salt stress reflected in phytochemical fingerprint and increase of shoots and roots biomass (by ca. 20% and 10%, respectively). The full quantitative and statistical evaluation of the metabolomic data lead to the detection of specialized metabolites that are possibly involved in stress response.
Acknowledgements: Study was supported by the Polish National Science Center postdoctoral grant” to S. Ślusarczyk (No. FUGA – DEC/2014/12/S/NZ9/00715) and the Ministry of Science and Higher Education special grant-in-aid for Botanical Garden #SPUB-2 – 2015.
Keywords: Medicinal plants, Perovskia, metabolomics, UPLC-TOF-MS, NMR.
 Ślusarczyk S, Topolski J, Domaradzki K, Adams M, Hamburger M, Matkowski A. Isolation and fast selective determination of nor-abietanoid diterpenoids from Perovskia atriplicifolia roots using LC-ESI-MS/MS with multiple reaction monitoring. Nat Prod Commun 2015; 10: 1149 – 1152
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