To evaluate the effects of orchid mycorrhizal fungi on the metabolites of Anoectochilus Formosanus Hayata by UHPLC-MS

Anoectochilus formosanus Hayata is used to treat the liver disorder, enhance memory with the pharmacological functions of anti-oxidation, anti-hyperliposis, lowering blood sugar, blood fat, anti-tumor, anti-inflammatory, anti-allergic, anti-virus in recent years. Orchid Mycorrhiza (Rhizoctonia solani) is recognized to promote the growth and increasing the absorption of minerals and nutrients, and to improve the ability of plants to resist the adverse environment and prevent other invasive pathogens for A. formosanus [1 – 3]. However, there is no discussion for the changes on the production of metabolites of A. formosanus when infected with R. solani under symbiotic condition. A. formosanus were selected with similar height and co-cultured with R. solani on 0.25% Oatmeal medium for 15, 30, 60 and 90 days. All of materials were harvested and extracted with MeOH, then applied into HPLC-UV and UHPLC-MS for the analysis of secondary metabolites. In this talk the growth rate and biomass of A. formosanus were increased when the plants co-cultured with R. solani under the symbiotic condition. The longer period for co-culture is better for biomass growth. There was 3 times on the biomass compared with control group after 3 months. In the other hand, the patterns of metabolites were total different between co-cultured system and control, either from leaves, stems and roots. It means the symbiotic culture system of A. formosanus with R. solani is able to influence the production of metabolites. After analysis by UHPLC-MS, the experimental group and the control group in the roots, stems, leaves of amino acids, chlorophyll and carbohydrates and lipids by SIMCA statistics that have significant differences. The determination of structures of characteristic components using UHPLC-MS/MS is under investigation.

Acknowledgements: Taipei Medical University Professor Ching-Kuo Lee all laboratory personnel.

Keywords: Anoectochilus formosanus Hayata, Orchid Mycorrhiza, Rhizoctonia solani, UHPLC-MS, Metabolites.

References:

[1] Chang CN, Chou LC. Growth responses, enzyme activities, and component changes as influenced by Rhizoctonia Orchid mycorrhiza on Anoectochilus formosanus Hayata. Botanical Studies 2007; 48: 445 – 451

[2] Cubeta MA, Echandi E, Gumpertz ML. Smith ECJ, Williamson EM, Wareham N, Kaatz GW, Gibbons S. Antibacterials and modulators of bacterial resistance from the immature cones of Chamaecyparis lawsoniana. Phytochemistry 2007; 68: 210 – 217

[3] Hadley LJ, Hardly EL. A check-list of mycorrhiza in the british flora. New Phytol 1987; 105:1 – 102