J reconstr Microsurg 2016; 32(08): 580-586
DOI: 10.1055/s-0036-1584205
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Salidroside Promotes Random Skin Flap Survival in Rats by Enhancing Angiogenesis and Inhibiting Apoptosis

Chen Deheng
1  Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
2  The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China
,
Zhou Kailiang
1  Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
2  The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China
,
Weng Weidong
1  Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
2  The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China
,
Jin Haiming
1  Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
2  The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China
,
Xu Daoliang
1  Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
2  The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China
,
Cai Ningyu
1  Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
2  The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China
,
Xu Huazi
1  Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
2  The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China
› Author Affiliations
Further Information

Publication History

12 January 2016

02 April 2016

Publication Date:
08 June 2016 (eFirst)

Abstract

Background Flap necrosis is frequently observed in flap transfer operations. Salidroside has been reported to reduce cell apoptosis by alleviating inflammation and oxidative stress. We investigated the effects of salidroside on the survival of random skin flaps.

Materials and Methods The McFarlane flap model was established in 80 rats that were divided into two groups and administered salidroside or saline solution intraperitoneally over 7 days. The area of necrosis and the extent of tissue edema were measured. Angiogenesis was assessed via lead oxide-gelatin angiography, immunohistochemistry for CD34, and VEGF expression. Cell apoptosis was evaluated by expression of cleaved caspase 3, caspase 3, Bax, and Bcl-2. The inflammatory response was evaluated using an ELISA kit for TNF-α and IL-6 in serum. Oxidative stress was assessed by measuring the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA).

Results Compared with controls, salidroside-treated flaps featured a greater area of surviving tissue and less edema. It also promoted the expression of VEGF and increased skin flap angiogenesis. Cell apoptosis, inflammation reaction, and oxidative stress were significantly attenuated in the salidroside group.

Conclusion Salidroside has a positive effect on improving random skin flap survival.