Planta Med 2015; 81 - PW_237
DOI: 10.1055/s-0035-1565861

Quantitative determination of protopine in Hypecoum procumbens subsp. atropunctatum

E Kaynar 1, A Emir 1, B Bozkurt 1, GI Kaya 1, MA Onur 1, NU Somer 1
  • 1Department of Pharmacognosy, Faculty of Pharmacy, Ege University 35100, Bornova, Izmir, Turkey

The genus Hypecoum L. (Papaveraceae) is represented by five species and two subspecies in the flora of Turkey [1]. Protopine, the main isoquinoline alkaloid found in Hypecoum species, has been shown to possess important biological activities [2]. In the present study, a reversed-phase HPLC-DAD method has been used for the quantitative determination of protopine in the aerial parts of Hypecoum procumbens L. subsp. atropunctatum Å. E. Dahl collected from four different localities in Western Turkey [3]. Standard sample of protopine was previously isolated in our laboratory and authenticated by spectral analysis. Quantitative determination was carried out by the external standard method based on peak areas. The linearity of the method was studied by injecting six known concentrations of protopine in the range of 15 – 120 µg mL-1. The calibration curve for protopine was determined as y = 22.15862x+4.16731. Validation studies were also carried out to show that the method is specific, accurate and precise. Limit of detection and limit of quantification were established at a signal-to-noise ratio (S/N) of 3 and 10, respectively. In the four specimens, the content of protopine ranged between 0.0604 – 0.1361%.

Acknowledgements: This study was financially supported by Ege University Research Fund (13/ECZ/001).

References:

[1] Özhatay N. Hypecoum L. In: Güner A, Özhatay N, Ekim T, Başer KHC editors. Flora of Turkey and the East Aegean Islands, Vol. 11, Edinburgh University Press; 2000: 20 – 22

[2] Wangchuk P, Keller PA, Pyne SG, Sastraruji T, Taweechotipatr M, Rattanajak R, Tonsomboon A, Kamchonwongpaisan S. Phytochemical and biological activity studies of the Bhutanese medicinal plant Corydalis crispa Nat Prod Commun 2012; 7: 575 – 580

[3] Gu Y, Qian D, Duan J, Wang Z, Guo J, Tang Y, Guo S. Simultaneous determination of seven main alkaloids of Chelidonium majus L. by ultraperformance LC with photodiode-array detection. J Sep Sci 2010; 33: 1004 – 1009