Planta Med 2015; 81 - PW_125
DOI: 10.1055/s-0035-1565749

In vivo analgesic activity and phytoconstituents of Scrophularia kotscyhana

G Renda 1, B Yaylı 1, M Sağlam 2, N Yazıcı 1, M Kadıoğlu 2, H Kırmızıbekmez 3
  • 1Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey
  • 2Department of Pharmacology, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon, Turkey
  • 3Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, 34755, Kayisdagi, İstanbul, Turkey

Scrophularia L. genus (Scrophulariaceae) is represented by 60 species (23 endemic) in Turkey [1]. Some Scrophularia species are used in different folk medicines for the treatment of many skin diseases, constipation, neuritis and bacterial-viral infections [2, 3]. In the present study we aimed to evaluate the analgesic activities of aerial parts and roots of Scrophularia kotscyhana Bentham separately. The plant materials were first extracted with MeOH. The MeOH extracts were dispersed in H2O and then subjected to partition with n-hexane, dichloromethane, ethyl acetate and n-butanol consecutively to obtain subextracts. Analgesic activities of all extracts at the doses of 5, 10, and 30 mg/kg (i.p.) were examined using hot plate test in mice. Diclofenac (15 mg/kg; i.p.) was used as reference analgesic agent. Among the tested extracts, MeOH extract prepared from the aerial parts and in the group of the subextracts prepared from the active crude MeOH extract, the n-butanol subextract showed analgesic activity both at the doses of 5, 10, and 30 mg/kg while n-hexane and dichloromethane subextracts displayed activity at 30 mg/kg. Phytochemical studies on n-butanol subextract led to the isolation of two new iridoid glycosides as an inseparable mixture along with two known compounds, β-Sitosterol 3-O-β-glucopyranoside and apigenin 7-O-β-D-glucopyranoside. The structures were identified on the basis of 1D- and 2D-NMR experiments as well as MS. The structures of the new compounds were elucidated as 8-O-acetyl-4'-O-(E)-p-coumaroylharpagide and 8-O-acetyl-4'-O-(Z)-p-coumaroylharpagide.

Acknowledgements: This research was supported by TUBITAK (SBAG-113S252).

References:

[1] Lall SS. Scrophularia L. In: Davis PH, ed. Flora of Turkey and the East Aegean Islands Vol 6. Edinburgh University Press; 1978; 603 – 647

[2] Duke JA, Ayensu ES. Medicinal Plants of China; 1985; 598 – 600 [3] Fernandez L et al. An iridoid diglycoside isolated from Scrophularia scorodonia. Phytochemistry 1995; 40: 1569 – 1571