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J Reconstr Microsurg 2015; 31(02): 132-138
DOI: 10.1055/s-0034-1395494
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Role of Anatomical Region and Hypoxia on Angiogenic Markers in Adipose-Derived Stromal Cells

Authors

  • Jacob Rinkinen

    1   Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan

  • Jeffrey Lisiecki

    1   Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan

  • Eboda Oluwatobi

    1   Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan

  • Jonathan Peterson

    1   Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan

  • Sara De La Rosa

    1   Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan

  • Kavitha Ranganathan

    1   Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan

  • Stewart C. Wang

    1   Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan

  • Paul S. Cederna

    1   Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan

  • Benjamin Levi

    1   Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan
Further Information

Publication History

25 March 2014

31 July 2014

Publication Date:
12 December 2014 (online)

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Abstract

Background Recent research into adipose-derived stem cells (ASCs) suggests that anatomical location has a major impact on the metabolic profile and differentiation capacity of ASCs. By having a better understanding of how various ASCs respond to cellular stressors such as hypoxia, which are induced during routine surgical procedures, we can facilitate future development of cell-based therapies to improve wound healing.

Patients and Methods Human ASCs were isolated from the superficial and deep adipose layers of four patients undergoing elective abdominoplasty. ASCs were cultured in hypoxic (1% O2, 5% CO2, and 94% N2) conditions. After 12 and 48 hours, ASCs were assessed for markers of angiogenesis by mRNA levels of vascular endothelial growth factor A (VEGF-A), vascular endothelial growth factor B (VEGF-B), and hypoxia inducible factor 1 α (HIF-1α). Western blot analysis was performed to assess levels of VEGF-A, p-NF-κB, and NF-κB. In addition, in vitro analysis of angiogenesis was performed using Matrigel assay (BD Biosciences, Franklin Lakes, NJ).

Results We observed significant increases in deep ASC's VEGF-A, VEGF-B, and HIF-1α mRNA expression compared with the superficial layer after 24-hour hypoxia (p < 0.05). Similar results were found when examining protein expression levels, with the deep ASCs expressing significantly larger amounts of VEGF-A and p-NF-κB (p < 0.05) compared with the superficial layer.

Conclusion Our results suggest that significant variations exist in the angiogenic profile of superficial and deep ASCs. We demonstrate that superficial ASCs are less prone to transcribe potent chemokines for angiogenesis, such as VEGF-A, VEGF-B, and HIF-1α and are less likely to translate VEGF-A and NF-κB. This may help with the selection of specific stem cell donor sites in future models for stem cell therapy.

Keywords

adipose-derived stromal cells - hypoxia - angiogenesis

Note

University of Michigan Institutional Review Board approval was obtained before commencement of the study (IRB # HUM00051190).