Analyse der Biofilmbildung auf kieferorthopädischen Apparaturen

Intraorale Biofilme auf kieferorthopädischen Apparaturen stellen eine Gefahr für die strukturelle Integrität der Hart- und Weichgewebe dar. Die Identifikation oraler Biofilme kann chemisch mittels der energiedispersiven Röntgenanalyse (EDX) und morphologisch mithilfe der Rasterelektronenmikroskopie wie der hochauflösenden Sekundärelektronenmikroskopie (SE) und der Rutherford-Backscattering-Detection (RBSD)-Methode erfolgen.

Unter Anwendung der beschriebenen Methodik lassen sich Prädilektionsstellen der Biofilmakkumulation unter Realbedingungen identifizieren und daraus notwendige Maßnahmen zur Oberflächenfunktionalisierung oder Gestaltoptimierung ableiten.

Intraoral biofilms on orthodontic appliances endanger the structural integrity of oral hard and soft tissues. Identification of oral biofilms can be performed chemically by energy dispersive X-ray spectroscopy (EDX) and morphologically by scanning electron microscopy by means of secondary electron microscopy (SE) and Rutherford-Backscattering-Detection (RBSD).

Predilection sites of biofilm accumulation can be identified by the above-mentioned methods under in-vivo conditions and information for surface functionalisation and shape optimisation and can be derived.

Schlüsselwörter

Biofilm - kieferorthopädische Apparatur - Rasterelektronenmikroskopie (REM) - energiedispersive Röntgenanalyse (EDX) - Flächenanalyse

Keywords

Biofilm - orthodontic appliances - scanning electron microscopy - energy dispersive X-ray spectroscopy (EDX) - surface analysis

• Literatur

• 1 Elter C, Heuer W, Demling A et al. Comparative analysis of biofilm formation on dental implant abutments with respect to supra- and subgingival areas: polytetrafluoroethylene versus titanium. Int J Prosthodont 2011; 24: 373-375
• 2 Fuchslocher Hellemann C, Grade S, Heuer W et al. Three-dimensional analysis of initial biofilm formation on polytetrafluoroethylene in the oral cavity. J Orofac Orthop 2013; 74: 458-467
• 3 Karp JM, Langer R. Development and therapeutic applications of advanced biomaterials. Curr Opin Biotechnol 2007; 18: 454-459
• 4 Hannig M. Transmission electron microscopic study of in vivo pellicle formation on dental restorative materials. Eur J Oral Sci 1997; 105: 422-433
• 5 Quirynen M, Bollen CM. The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man. A review of the literature. J Clin Periodontol 1995; 22: 1-14
• 6 Hannig M, Döbert A, Stigler R et al. Initial salivary pellicle formation on solid substrates studied by AFM. J Nanosci Nanotechnol 2004; 4: 532-538
• 7 Scheie AA. Mechanisms of dental plaque formation. Adv Dent Res 1994; 8: 246-253
• 8 Bos R, van der Mei HC, Busscher HJ. Physico-chemistry of initial microbial adhesive interactions--its mechanisms and methods for study. FEMS Microbiol Rev 1999; 23: 179-230
• 9 Costerton JW, Lewandowski Z, Caldwell DE et al. Microbial biofilms. Annu Rev Microbiol 1995; 49: 711-745
• 10 Hall-Stoodley L, Costerton JW, Stoodley P. Bacterial biofilms: from the natural environment to infectious diseases. Nat Rev Microbiol 2004; 2: 95-108
• 11 Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet 2001; 358: 135-138
• 12 Teughels W, Van Assche N et al. Effect of material characteristics and / or surface topography on biofilm development. Clin Oral Implants Res 2006; 17 (Suppl. 02) 68-81
• 13 Lee SM, Yoo SY, Kim HS et al. Prevalence of putative periodontopathogens in subgingival dental plaques from gingivitis lesions in Korean orthodontic patients. J Microbiol 2005; 43: 260-265
• 14 Paolantonio M, di Girolamo G, Pedrazzoli V et al. Occurrence of Actinobacillus actinomycetemcomitans in patients wearing orthodontic appliances. A cross-sectional study. J Clin Periodontol 1996; 23: 112-118
• 15 Atack NE, Sandy JR, Addy M. Periodontal and microbiological changes associated with the placement of orthodontic appliances. A review. J Periodontol 1996; 67: 78-85
• 16 van Gastel J, Quirynen M, Teughels W et al. Longitudinal changes in microbiology and clinical periodontal variables after placement of fixed orthodontic appliances. J Periodontol 2008; 79: 2078-2086
• 17 Naranjo AA, Triviño ML, Jaramillo A et al. Changes in the subgingival microbiota and periodontal parameters before and 3 months after bracket placement. Am J Orthod Dentofacial Orthop 2006; 130: 217-222
• 18 Sallum EJ, Nouer DF, Klein MI et al. Clinical and microbiologic changes after removal of orthodontic appliances. Am J Orthod Dentofacial Orthop 2004; 126: 363-366
• 19 Øgaard B. Prevalence of white spot lesions in 19-year-olds: a study on untreated and orthodontically treated persons 5 years after treatment. Am J Orthod Dentofacial Orthop 1989; 96: 423-427
• 20 Hobbs CP, McMillan JW, Palmer DW. The effects of surface topography in nuclear microprobe Rutherford backscattering analysis. Nucl Instr Meth Phys Res 1988; 30: 342-348
• 21 Goldstein J, Newbury DE, Joy DC et al. Scanning electron microscopy and x-ray microanalysis. New York: Kluwer Academic, Plenum Publishers; 2003: 297-353
• 22 Elter C, Heuer W, Demling A et al. Supra- and subgingival biofilm formation on implant abutments with different surface characteristics. Int J Oral Maxillofac Implants 2008; 23: 327-334
• 23 Heuer W, Elter C, Demling A et al. Analysis of early biofilm formation on oral implants in man. J Oral Rehabil 2007; 34: 377-382
• 24 Demling A, Elter C, Heidenblut T et al. Reduction of biofilm on orthodontic brackets with the use of a polytetrafluoroethylene coating. Eur J Orthod 2010; 32: 414-418
• 25 Demling A, Heuer W, Elter C et al. Analysis of supra- and subgingival long-term biofilm formation on orthodontic bands. Eur J Orthod 2009; 31: 202-206