Abstract
Three-dimensional (3D) histology provides a complete 3D representation of the margins
of a tumor specimen on histological slides. This eliminates diagnostic gaps and provides
maximum sensitivity in identifying tumor outgrowths. The method is used to confirm
tumor-free margins or, in case of an incomplete tumor resection, to make even very
small infiltrated areas visible with topographic orientation. The basic principle
of 3D histology is the conversion from the 3D structure of the tumor-specimen margins
into a two-dimensional view of histological sections. The 3D margins are flattened
with their outside down and then cut into pieces, suitable for histopathological procedures
of cryo or paraffin sections. The management of a full 3D histology needs a tight
communication between the surgeon and the pathologist. Clear and safe rules are necessary
to enable both the surgeon and the pathologist to point out areas of incomplete tumor
excisions very precisely. Using a circle as representation of the separation between
tumor base and margin, the pathologist can point out the exact position of an outgrowth
by referring to different positions on the circle in clock times. This system will
be sufficient to point out tumor outgrowths. The advantage is that it is very simple
to communicate and easy implemented in any electronic communication.
Keywords
malignant skin tumors - 3D histology - Mohs micrographic surgery - rules for embedding
- surgical margins - tumescent local anesthesia
