Planta Med 2013; 79 - PL38
DOI: 10.1055/s-0033-1348679

Two New Metabolites from Saccharicola sp. an Endophytic Fungus in Eugenia jambolana (Myrtaceae)

AR Araujo 1, VM Chapla 1, MN Lopes 1, DHS Silva 1, AJ Cavalheiro 1, V da S Bolzani 1
  • 1Nuclei of Bioassays, Biosynthesis and Ecophysiology of Natural Products, NuBBE, São Paulo State University-UNESP, Chemistry Institute-14.800 – 900, Araraquara, SP, Brazil, Maria C. Marx Young
  • 2Secção de Fisiologia e Bioquímica de Plantas, IBt, CP 4005, CEP 01061, 970, São Paulo, SP, Brazil

The genus Eugenia, comprising about 500 species widely distributed worldwide is well known for its diverse biological and pharmacological properties.1 Eugenia jambolana Lam. (Syn. Syzygium cumini Skeels or Syzynium jambolana DC) has been used in traditional Brazilian medicine for the treatment of diabetes. This observation prompted us to launch a program aiming to search novel bioactive metabolites from cultures of endophytes colonized inside E. jambolana. Stems and fruits of E. jambolana, were submitted to isolation of endophytic fungi and 13 isolates were obtained, preserved and cultivated on liquid medium (Czapek) to get their crude extracts. The strain Saccharicola sp. isolated from stems was selected for chemical and biological investigation because of the significant antifungal activity against the phytopatogenic fungi Cladosporium cladosporioides and C. sphaerspermum, as well as AChE inhibitor activitiy. Fractionation of the crude AcOEt extract by flash chromatography column on reversed-phase C-18 silica, followed by reversed-phase HPLC, afforded two new compounds 1 – 2. The structures were established conclusively by IR, MS and extensive 1D and 2D NMR spectra analysis.

1VASI, S.; AUSTIN, A. Antioxidant potential of Eugenia jambolana Lam. Seeds. J. Biol. Sci., vol. 9, n. 8, p. 894 – 898, 2009.

Acknowledgements: FAPESP, CAPES and CNPq