Planta Med 2013; 79 - PL32
DOI: 10.1055/s-0033-1348673

Production of Cytotoxic Glidobactins/Luminmycins by Photorhabdus asymbiotica in Liquid Media and Live Crickets

CM Theodore 1, JB King 1, J You 1, RH Cichewicz 1
  • 1Natural Products Discovery Group, Institute for Natural Products Applications and Research Technologies, Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 – 5251, United States

Photorhabdus asymbiotica has a unique, biphasic lifestyle as both a symbiotic and pathogenic bacterium. Genomic analysis revealed several synthetic gene clusters capable of producing secondary metabolites however recent reports indicate several of these clusters are silent under laboratory culture conditions. Through media and culture condition manipulation, we have successfully induced the production of new and known glidobactin/luminmycin derivatives from P. asymbiotica. Metabolite production was also induced through culturing the bacterium in live crickets