Effects of a Moderate Physical Training on the Leptin Synthesis by Adipose Tissue of Adult Rats Submitted to a Perinatal Low-protein Diet

I.H. P. de Mélo Montenegro; L. Moita; F.K. W. dos Reis; E. de Oliveira; P. C. Lisboa; E. G. de Moura; R. Manhães-de-Castro; C. G. Leandro

doi:10.1055/s-0032-1316350

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2012; 44(11): 814-818
DOI: 10.1055/s-0032-1316350

Original Basic

Effects of a Moderate Physical Training on the Leptin Synthesis by Adipose Tissue of Adult Rats Submitted to a Perinatal Low-protein Diet

I.H. P. de Mélo Montenegro

¹Department of Nutrition, Federal University of Pernambuco, Recife, Pernambuco, Brazil

,

L. Moita

¹Department of Nutrition, Federal University of Pernambuco, Recife, Pernambuco, Brazil

,

F.K. W. dos Reis

¹Department of Nutrition, Federal University of Pernambuco, Recife, Pernambuco, Brazil

,

E. de Oliveira

²Department of Physiological Science, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil

,

P. C. Lisboa

²Department of Physiological Science, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil

,

E. G. de Moura

²Department of Physiological Science, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil

,

R. Manhães-de-Castro

¹Department of Nutrition, Federal University of Pernambuco, Recife, Pernambuco, Brazil

,

C. G. Leandro

³Department of Physical Education and Sports Science, CAV, Federal University of Pernambuco, Vitória de Santo Antão, Pernambuco, Brazil

› Author Affiliations

Abstract

The aim of the study was to verify if moderate physical training affects leptin content in visceral and subcutaneous adipose tissue of adult rats subjected to a low-protein diet during the perinatal period. Male Wistar rats were divided into 2 groups according to their mother’s diet during gestation and lactation: control (17% casein, C, n=12) and low-protein (8% casein, LP, n=12). On postnatal day 60, half of each group was submitted to moderate physical training (8 wks, 5 d · wk^− 1, 60 min · d^− 1, at 70% of VO_2max, T) or not. After the physical training period, visceral and subcutaneous adipose tissues were removed. Leptin content was evaluated by western blotting. Starting from the fifth week on, T pups showed a reduction in the body weight. Similarly, LP+T offspring showed a lower body weight starting from the sixth week on. Western blotting analysis showed that leptin content in the visceral tissue was higher in the LP rats (p<0.01) and it was reversed in LP+T. No difference was found in the subcutaneous tissue. Moderate physical training attenuated the effects of a perinatal low-protein diet on the leptin content in visceral adipose tissue in adult offspring.

Key words

perinatal undernutrition - physical exercise - programming - leptin - visceral adipose tissue

Full Text

References

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