Am J Perinatol 2012; 29(08): 615-622
DOI: 10.1055/s-0032-1311986
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

The Effects of Prostaglandin E1 and Prostaglandin E2 on in vitro Myometrial Contractility and Uterine Structure

Giuseppe Chiossi
1  Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas
,
Maged M. Costantine
1  Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas
,
Egle Bytautiene
1  Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas
,
Talar Kechichian
1  Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas
,
Gary D.V. Hankins
1  Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas
,
Elena Sbrana
1  Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas
,
George R. Saade
1  Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas
,
Monica Longo
1  Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas
› Author Affiliations
Further Information

Publication History

27 November 2011

24 January 2012

Publication Date:
25 May 2012 (eFirst)

Abstract

Objective To estimate the effects of prostaglandin E1 (PGE1) and E2 (PGE2) on myometrial contractility and structure in vitro.

Study Design Myometrial strips from 18 women were incubated with PGE1 (10−5 mol/L), PGE2 (10−5 mol/L), or solvent (CTR) for up to 360 minutes in organ chambers for isometric tension recording. The area under the contraction curve, total collagen content, and percentage of the area covered by connective tissue were calculated at various time periods.

Results PGE1 significantly increased in vitro myometrial contractility up to 90 minutes when compared with PGE2 and CTR (p < 0.01) and up to 180 minutes as compared with PGE2 (p < 0.05). After 360 minutes, CTR and PGE1 samples had lower total collagen content and area covered by connective tissue than PGE2 (p < 0.01).

Conclusion The effects of prostaglandins on the uterus cannot be solely explained by contractility. Treatment with PGE1 significantly increased myometrial contractions and decreased both total collagen content and the area covered by connective tissue. Such findings may explain the higher rates of vaginal delivery, tachysystole, and uterine rupture associated with PGE1 use.