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Horm Metab Res 2012; 44(10): 786-789
DOI: 10.1055/s-0032-1311631
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

PDE4 Control on cAMP/PKA Compartmentation Revealed by Biosensor Imaging in Neurons

P. Vincent
1   Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France
2   Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France
,
L.R. V. Castro
1   Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France
2   Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France
,
N. Gervasi
1   Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France
2   Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France
3   Current Address: Inserm UMR-S 839, Institut du Fer à Moulin, Paris, France
,
E. Guiot
1   Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France
2   Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France
,
M. Brito
1   Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France
2   Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France
,
D. Paupardin-Tritsch
1   Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France
2   Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France
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Publikationsverlauf

received 11. Januar 2012

accepted 05. April 2012

Publikationsdatum:
11. Mai 2012 (online)

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Abstract

Electrophysiological recordings (using the slow-AHP potassium current) together with novel biosensor imaging methods (with AKAR and Epac sensors) were used in preparations of rodent brain slices to record PKA activation in real time and in individual neurons. The experiments revealed the propagation of the PKA signal from the membrane to the cytosol and eventually to the nucleus. The experiments show how the geometry of the neurons combined with phosphodiesterase activities (mostly rolipram-sensitive PDE4) contributes to a functional compartmentation of the cAMP in subcellular domains.

Key words

imaging - phosphodiesterase - protein kinase - cyclic AMP - neuron - cortex
 

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