PDE4 Control on cAMP/PKA Compartmentation Revealed by Biosensor Imaging in Neurons

P. Vincent; L.R. V. Castro; N. Gervasi; E. Guiot; M. Brito; D. Paupardin-Tritsch

doi:10.1055/s-0032-1311631

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2012; 44(10): 786-789
DOI: 10.1055/s-0032-1311631

Original Basic

PDE4 Control on cAMP/PKA Compartmentation Revealed by Biosensor Imaging in Neurons

P. Vincent

¹Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France

²Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France

,

L.R. V. Castro

¹Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France

²Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France

,

N. Gervasi

¹Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France

²Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France

³Current Address: Inserm UMR-S 839, Institut du Fer à Moulin, Paris, France

,

E. Guiot

¹Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France

²Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France

,

M. Brito

¹Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France

²Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France

,

D. Paupardin-Tritsch

¹Centre National de la Recherche Scientifique, Unité Mixe de Recherche UMR7102, Paris, France

²Université Pierre et Marie Curie, UPMC, Unité Mixe de Recherche UMR7102, Paris, France

› Author Affiliations

Abstract

Electrophysiological recordings (using the slow-AHP potassium current) together with novel biosensor imaging methods (with AKAR and Epac sensors) were used in preparations of rodent brain slices to record PKA activation in real time and in individual neurons. The experiments revealed the propagation of the PKA signal from the membrane to the cytosol and eventually to the nucleus. The experiments show how the geometry of the neurons combined with phosphodiesterase activities (mostly rolipram-sensitive PDE4) contributes to a functional compartmentation of the cAMP in subcellular domains.

Key words

imaging - phosphodiesterase - protein kinase - cyclic AMP - neuron - cortex

Full Text

References

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