ABSTRACT
Osteoarthritis (OA) is a disease that commonly affects human and veterinary patients.
Animal models are routinely used for OA research, and the dog is a nearly ideal species
for translational investigation of human OA biomarkers. The cytokine, chemokine, and
matrix metalloprotease (MMP) profiles of synovial fluid, serum, and urine from dogs
with surgically induced and naturally occurring OA were compared with dogs without
OA using xMAP technology (Qiagen Inc., Valencia, CA). Markers that exhibited significant
differences between groups were identified (monocyte chemoattractant protein 1 [MCP1],
interleukin 8 [IL8], keratinocyte-derived chemoattractant [KC], and MMP2 and MMP3),
and their sensitivities and specificities were calculated to determine their diagnostic
usefulness in a future biomarker panel. Synovial fluid IL8 was the most sensitive,
but MCP1 was also highly sensitive and specific. The alterations in KC suggested that
it may differentiate between cruciate disease and other types of OA, and the MMPs
were most sensitive and specific in the serum. This study provided additional insight
to the participation of cytokines, chemokines, and MMPs in OA, and potential diagnostic
biomarker candidates were identified. A brief literature review of other biomarker
candidates previously examined using animal models is discussed.
KEYWORDS
Osteoarthritis - biomarker - chemokine - matrix metalloprotease
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Bridget GarnerD.V.M. Ph.D.
Department of Veterinary Pathology, University of Georgia
501 DW Brooks Drive, Athens, GA 30605
Email: garnerb@uga.edu
