Arzneimittelforschung 2008; 58(7): 336-341
DOI: 10.1055/s-0031-1296516
Liver Therapeutics
Editio Cantor Verlag Aulendorf (Germany)

Simultaneous Determination of 6,7-Dimethylesculetin and Geniposide in Rat Plasma and its Application to Pharmacokinetic Studies of Yin Chen Hao Tang Preparation

Xijun Wang
1  Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Haitao Lv
1  Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Hui Sun
1  Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Wenjun Sun
1  Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Lian Liu
1  Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Ping Wang
1  Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, P. R. China
,
Hongxin Cao
2  China Academy of Chinese Medical Science, Beijing, P. R. China
› Author Affiliations
Further Information

Publication History

Publication Date:
15 December 2011 (online)

Abstract

A method for the rapid and simultaneous determination of 6,7-dimethylesculetin (CAS 120-08-1) and geniposide (CAS 24512-63-8) in rat plasma has been developed, using validated high performance liquid chromatography (HPLC) with solid phase extraction (SPE). The HPLC analysis was performed on a commercially available column (200 mm × 4.6 mm, 5 µm) with acetonitrile-methanol −0.1% aqueous formic acid as mobile phase and the UV detection at 343 nm and 238 nm for 6,7-dimethylesculetin and geniposide, respectively. The calibration curves for 6,7-dimethylesculetin and geniposide were linear over the range 0.4–25.6 µg/mL and 1.12–71.68 µg/mL, respectively. The lower limits of quantitation were 0.40 µg/mL and 1.12 µg/mL, and the lower limits of detection were 0.06 µg/mL and 0.09 µg/mL, respectively. The intra-day and inter-day precision for 6,7-dimethylesculetin and geniposide were < 5%, whereas the absolute recovery percentages were > 74%. A successful application of the developed HPLC analysis was demonstrated for the pharmacokinetic study of a Traditional Chinese Medicine formula of Yin Chen Hao Tang preparation.