Effect of Exercise on Vascular Superoxide Dismutase Expression in High-Risk Pregnancy

ABSTRACT

Endothelial dysfunction of the maternal vasculature induced by pro-oxidants may contribute to the development of preeclampsia. Obesity results in vascular inflammation and oxidative stress and is therefore a risk factor for preeclampsia. Regular exercise is known to induce antioxidants. We recently demonstrated that stretchers (subjects who performed low-intensity exercises) had a lower incidence of preeclampsia as opposed to walkers (moderate-intensity exercise; 2.6% versus 14.6%). We now seek to determine the possible protective mechanisms. We hypothesized that stretchers will have higher vascular levels of the antioxidant superoxide dismutase (SOD) and plasma transferrin levels, an antioxidant marker. We conducted immunohistochemical analyses of blood vessels embedded in fat biopsy samples obtained during cesarean sections from women who were randomized to either stretching (n = 6) or walking (n = 5) exercises. In addition, levels of plasma transferrin were measured. SOD expression was increased (p < 0.05) in stretchers [106.3 (interquartile range 84.2 to 127.8 arbitrary units (AU)] when compared with that of walkers [56.92 (interquartile range 46.35 to 82.32 AU)]. Transferrin levels continued to increase throughout gestation only among the stretchers. There appears to be a higher antioxidant protective effect in subjects who performed low-intensity exercise during pregnancy.

Preeclampsia - superoxide dismutase - oxidative stress - transferrin - endothelial dysfunction

REFERENCES

• 1 Crane J, Delaney T. Diagnosis, evaluation, and management of the hypertensive disorders of pregnancy.  J Obstet Gynaecol Can. 2008;  30 561 author reply 561-562
• 2 Roberts J M. Endothelial dysfunction in preeclampsia.  Semin Reprod Endocrinol. 1998;  16 5-15
• 3 Redman C W, Sargent I L. Immunology of pre-eclampsia.  Am J Reprod Immunol. 2010;  63 534-543
• 4 Sankaralingam S, Arenas I A, Lalu M M, Davidge S T. Preeclampsia: current understanding of the molecular basis of vascular dysfunction.  Expert Rev Mol Med. 2006;  8 1-20
• 5 Granger J P, Alexander B T, Llinas M T, Bennett W A, Khalil R A. Pathophysiology of preeclampsia: linking placental ischemia/hypoxia with microvascular dysfunction.  Microcirculation. 2002;  9 147-160
• 6 Yeo S, Davidge S T. Possible beneficial effect of exercise, by reducing oxidative stress, on the incidence of preeclampsia.  J Womens Health Gend Based Med. 2001;  10 983-989
• 7 Carlsohn A, Rohn S, Bittmann F, Raila J, Mayer F, Schweigert F J. Exercise increases the plasma antioxidant capacity of adolescent athletes.  Ann Nutr Metab. 2008;  53 96-103
• 8 Fisher-Wellman K, Bloomer R J. Acute exercise and oxidative stress: a 30 year history.  Dyn Med. 2009;  8 1
• 9 Saftlas A F, Logsden-Sackett N, Wang W, Woolson R, Bracken M B. Work, leisure-time physical activity, and risk of preeclampsia and gestational hypertension.  Am J Epidemiol. 2004;  160 758-765
• 10 Sorensen T K, Williams M A, Lee I M, Dashow E E, Thompson M L, Luthy D A. Recreational physical activity during pregnancy and risk of preeclampsia.  Hypertension. 2003;  41 1273-1280
• 11 Hernández R, Mahedero G, Caballero M J et al.. Effects of physical exercise in pre-and postmenopausal women on lipid peroxidation and antioxidant systems.  Endocr Res. 1999;  25 153-161
• 12 Ji L L, Leeuwenburgh C, Leichtweis S et al.. Oxidative stress and aging. Role of exercise and its influences on antioxidant systems.  Ann N Y Acad Sci. 1998;  854 102-117
• 13 Østerdal M L, Strøm M, Klemmensen A K et al.. Does leisure time physical activity in early pregnancy protect against pre-eclampsia? Prospective cohort in Danish women.  BJOG. 2009;  116 98-107
• 14 Roggensack A M, Zhang Y, Davidge S T. Evidence for peroxynitrite formation in the vasculature of women with preeclampsia.  Hypertension. 1999;  33 83-89
• 15 Gutteridge J M, Quinlan G J. Antioxidant protection against organic and inorganic oxygen radicals by normal human plasma: the important primary role for iron-binding and iron-oxidising proteins.  Biochim Biophys Acta. 1993;  1156 144-150
• 16 Hubel C A, Kozlov A V, Kagan V E et al.. Decreased transferrin and increased transferrin saturation in sera of women with preeclampsia: implications for oxidative stress.  Am J Obstet Gynecol. 1996;  175 (3 Pt 1) 692-700
• 17 Sankaralingam S, Xu H, Jiang Y, Sawamura T, Davidge S T. Evidence for increased methylglyoxal in the vasculature of women with preeclampsia: role in upregulation of LOX-1 and arginase.  Hypertension. 2009;  54 897-904
• 18 Sankaralingam S, Xu Y, Sawamura T, Davidge S T. Increased lectin-like oxidized low-density lipoprotein receptor-1 expression in the maternal vasculature of women with preeclampsia: role for peroxynitrite.  Hypertension. 2009;  53 270-277
• 19 Sankaralingam S, Xu H, Davidge S T. Arginase contributes to endothelial cell oxidative stress in response to plasma from women with preeclampsia.  Cardiovasc Res. 2010;  85 194-203
• 20 Yeo S, Davidge S, Ronis D L, Antonakos C L, Hayashi R, O'Leary S. A comparison of walking versus stretching exercises to reduce the incidence of preeclampsia: a randomized clinical trial.  Hypertens Pregnancy. 2008;  27 113-130
• 21 Yeo S. Adherence to walking or stretching, and risk of preeclampsia in sedentary pregnant women.  Res Nurs Health. 2009;  32 379-390
• 22 Yeo S. A randomized comparative trial of the efficacy and safety of exercise during pregnancy: design and methods.  Contemp Clin Trials. 2006;  27 531-540
• 23 Kaiser P S, Kirby R S. Obesity as a risk factor for cesarean in a low-risk population.  Obstet Gynecol. 2001;  97 39-43
• 24 Stepan H, Scheithauer S, Dornhöfer N, Krämer T, Faber R. Obesity as an obstetric risk factor: does it matter in a perinatal center?.  Obesity (Silver Spring). 2006;  14 770-773
• 25 Friedman J M. Obesity in the new millennium.  Nature. 2000;  404 632-634
• 26 Kopelman P G. Obesity as a medical problem.  Nature. 2000;  404 635-643
• 27 Meijer E P, Goris A H, van Dongen J L, Bast A, Westerterp K R. Exercise-induced oxidative stress in older adults as a function of habitual activity level.  J Am Geriatr Soc. 2002;  50 349-353
• 28 Sentürk U K, Gündüz F, Kuru O et al.. Exercise-induced oxidative stress leads hemolysis in sedentary but not trained humans.  J Appl Physiol. 2005;  99 1434-1441
• 29 Avery M D, Walker A J. Acute effect of exercise on blood glucose and insulin levels in women with gestational diabetes.  J Matern Fetal Med. 2001;  10 52-58
• 30 Manders R J, Van Dijk J W, van Loon L J. Low-intensity exercise reduces the prevalence of hyperglycemia in type 2 diabetes.  Med Sci Sports Exerc. 2010;  42 219-225
• 31 Leaf D A, Kleinman M T, Hamilton M, Deitrick R W. The exercise-induced oxidative stress paradox: the effects of physical exercise training.  Am J Med Sci. 1999;  317 295-300