Antigenotoxic study of rice extracts from Thai Sung-Yod red rice cultivar in human lymphocytes in vitro

The antigenotoxic effect of rice extracts from Sung-Yod red rice cultivar produced in Thailand have been investigated in human lymphocytes for a sister chromatid exchange (SCE) assay in vitro. Pretreatment of 2 different fractions of the rice extracts, the rinsed water fraction (SYGW) and the aqueous extract of freeze dried boiled rice bran (SYBBOIL) at the concentrations of 6.25–100µg/ml alone for 2h followed by 0.1µg/ml doxorubicin as chemotherapeutic agent for 2h could not significantly reduce SCE level induced by doxorubicin (p<0.05). Our data indicated that rice extracts in the form of SYGW and SYBBOIL fractions can not protect human lymphocytes from genotoxic damage induced by 0.1µg/ml doxorubicin. In conclusion, our Sung-Yod rice extracts in the form of SYGW and SYBBOIL have no antigenotoxic potential against doxorubicin. However, their antigenotoxic potential is further investigated using other genotoxic compounds to investigate its benefit as herbal medicine.

Acknowledgements: This study was supported by Research Fund, National Research Council of Thailand (NRCT)

References: 1. Phutthaphadoong, S. et al. (2010) Oncol.Rep. 23: 53–59.

2. Kannan A. et.al. (2008)J Agric Food Chem 56:11643–7.

3. Nam, SH. et al. (2005)J Agric Food Chem. 53:816–22.