Quantification of some phenolic acid and rutin in the leaves of rosemary from Turkey and Croatia

Rosemary contains a number of potentially biologically active compounds, such as carnosic acid and rosmarinic acid (RA), camphor, caffeic acid (CA), ursolic acid, betulinic acid, rosmaridiphenol and rosmanol. OBJECTIVES: In this study, using HPLC-ED system, analysis of gallic acid (GA), RA, CA, and rutin was carried out in water extracts of rosemary. Methods: Analyses of GA, RA, CA and rutin were performed of leaves rosemary. The drug (1g) was powdered and extracted with pure water (9ml). Afterward 1ml of that extract was decanted and centrifuged. Supernatant was used for analysis. The standard solutions were of GA, RA, and CA dissolved in mobile phase, and rutin was dissolved pure water. HPLC conditions were following: Mobile phase methanol-acetonitrile-water-acetic acid (20+10+70+1); ED detector with range 50nA, potential +0.840 V, filter 0.02Hz; flow rate 1ml/min; temperature 25°C, Column: ODS hypersil. Results: The content in (mg/g) of GA was in the rosemary from Turkey 1.54, RA 11.31, CA 1.12, and rutin 2.45. The content in (mg/g) of GA was in the rosemary from Croatia 0.77, RA 14.62, CA 0.96, and rutin 1.61. Conclusion: The highest content of RA was found in leaves of rosemary from Croatia, and highest content of GA, CA and rutin was found in leaves of rosemary from Turkey.