Planta Med 2009; 75 - PG3
DOI: 10.1055/s-0029-1234657

Chemical composition of water extract of Gnaphalium uliginosum. Part 2

Y Kolesnik 1, E Titova 1, V Chertkov 2, A Shestakova 3, V Tikhonov 1, D Shmatkov 1
  • 1Diod Co. 1, 11A, Derbenevskaya Str., Moscow, 115114, Russia
  • 2M.V.Lomonosov Moscow State University 2, Leninskie Gory, Moscow, 119992, Russia
  • 3State Research Institute of Chemistry and Technology of Organoelement Compounds, 3, Moscow, 111123, Russia

Water extract of Gnaphalium uliginosum is used in Russia as hypotensic. The analysis of the dried water extract by RP-HPLC (Fig.1) shows that there are two groups of peaks for compounds differing in lipophylicity: 1–6 and 7–10. In recent study [1] we reported the composition of the first part (1–6) of extract. It was established that all substances belong to a general class of hydroxycinnamic acids. Current work is devoted to the structure estimation for main components of the second (more hydrophobic) part of this extract. Using SPE, analytical and preparative HPLC, UV and high resolution NMR spectroscopy, we found that the more hydrophobic group of compounds contains: 1,3,4,5-tetra-hydroxycyclohexanecarboxylic acid-bis-4,5-(3,4-dihydroxycinnamate) (7); 1,3,4,5-tetra-hydroxycyclohexanecarboxylic acid-bis-3,5-(3,4-dihydroxycinnamate) (8); 1,3,4,5-tetrahydroxy-cyclohexanecarboxylic acid-bis-3,4-(3,4-dihydroxycinnamate) (9) and 1,3,4,5-tetra-hydroxycyclohexanecarboxylic acid-tris-1,3,5-(3,4-dihydroxycinnamate) (10).

Fig.1. RP-HPLC of dried water extract of Gnaphalium uliginosum.

Reference: [1] Kolesnik, Y. et al. (2008) Planta Med. 74:1095.