Pseudojujubogenin glycosides from in vitro regenerated Bacopa monnieri (L.) Wettst

Bacopa monnieri (L.) Wettst. (Scrophulariaceae) is a medicinal plant used in Ayurvedic system to improve intelligence and memory [1, 2]. B. monnieri was studied in vitro on Murashige and Skoog (MS) medium supplemented with different auxins and cytokinins. Pseudojujubogenin glycosides contents were determined by ELISA using bacopaside I polyclonal antibodies [3]. Stem explants cultured on a medium containing thidiazuron (TDZ) at 0.1mg/l resulted in the highest number of shoot (117 shoots/explant). Regenerated plants from a medium with TDZ at 0.5mg/l contained the highest level of pseudojujubogenin glycosides (30.62±1.29mg/g dry wt.). Plantlets regeneration from TDZ at 0.1mg/l showed 83.33% survival rate in the field and contained pseudojujubogenin glycosides in concentration of 29.65±2.92mg/g dry wt. On the other hand, plantlets from TDZ at 0.5mg/l showed a 50% survival rate in the field and contained more pseudojujubogenin glycosides (43.87±4.39mg/g dry wt). The effect of an elicitor on pseudojujubogenin glycosides content in B. monnieri whole plant cultures was investigated. Yeast extract at 2mg/ml gave the highest concentration of pseudojujubogenin glycosides (41.38±2.12mg/g dry wt) after 5 days which was 1.7 fold higher than in the control cultures. Chitosan at 150mg/l also increased pseudojujubogenin glycosdies production (40.83±2.24mg/g dry wt) after 5 days of culture.

Acknowledgements: NRCT-JSPS joint research program, Thailand-Japan.

References: 1. Stough. et al. (2001) Psychopharmacology 156: 481–484.

2. Roodenrys et al. (2002) Neuropsychopharmacology 27: 279–281.

3. Phrompittayarat et al. (2007) Anal Chim Acta 584: 1–6.