Planta Med 2008; 74 - PG25
DOI: 10.1055/s-0028-1084778

Salicylic acid elicitation induces erythrinane alkaloids accumulation in suspension culture of Erythrina americana Miller

R San Miguel-Chávez 1, M Soto-Hernández 1, AC Ramos-Valdivia 2, G Kite 3
  • 1Orientación en Botánica, Campus Montecillo, Colegio de Postgraduados, Montecillo 56230, México
  • 2Departamento de Biotecnología, Centro de Investigación y de Estudios Avanzados del IPN, Av. Politécnico Nacional 2508, 07360, México
  • 3Royal Botanic Garden, Kew Richmound, UK

Our on going research on Erythrina alkaloids has demonstrated their potential applications in several areas of research and this has justified to look for improved sources of them. In this paper we searched the alkaloid production under elicitation of the cell suspension culture of E. americana and 10-10, 10-12 and 10-14 M salicylic acid (SA) was added 7 days after subculture.

The results showed considerable variations in alkaloid accumulation reaching maximum in the samples grown with 10-14 M SA, it was observed that erysodine, α-erythroidine and erysotrine were those responded to these treatments in amounts of 50, 26.2 and 21.75µg g-1 dry weight respectively, whereas the controls of these alkaloids were 10 times lesser. This response is according with several reports [2,3], because SA added into the medium favored the presence of the alkaloids.

References: 1. Gatto, et al. (2004) CNS Drug Rev. 10:147–166.

2. Sudha, et al. (2003) Curr Sci 85: 1212–1217.

3. Kang, S. et al. (2004) Plant Sci 166(3): 745–751.