Download PDF
Synfacts 2008(11): 1213-1213  
DOI: 10.1055/s-0028-1083392
Organo- and Biocatalysis
© Georg Thieme Verlag Stuttgart ˙ New York

Synthesis of (R)-Pantolactone

Contributor(s): Benjamin List, Frank Lay
B. Pscheidt, Z. Liu, R. Gaisberger, M. Avi, W. Skranc, K. Gruber, H. Griengl, A. Glieder*
Research Centre Applied Biocatalysis GmbH, Graz, Graz University of Technology, and DSM Fine Chemicals Austria Nfg. GmbH & Co KG, Linz, Austria
Further Information

Publication History

Publication Date:
23 October 2008 (online)

    Permissions and Reprints

Significance

A pH-stable hydroxynitrile lyase (HNL) for the conversion of hydroxypivaldehyde (1) and pivaldehyde was developed by screening for stereoselective cyanohydrin synthesis in 96 well plates. Stepwise redesign of PaHNL5 gene and enzyme leads to elevated amounts of a highly efficient biocatalyst for (R)-pantolactone (2) synthesis (er = 98.5:1.5, 100% conversion) .