Bone and muscle development in three inbred female mouse strains

ABSTRACT

Muscle force is thought to be one of the main determinants of bone development. Hence, peak muscle growth is expected to precede peak bone growth. In this study, we investigated muscle and bone development in female C57BL/6 J, DBA/2JRj, and C3H/HeOuJ mice. Femoral cortical and trabecular bone structure and the weights of selected muscles were assessed at the ages of 8, 16, and 24 weeks. Muscle mass increased from 8 to 24 weeks in all 3 strains, suggesting peak muscle development at 24 weeks or later. Bone volume fraction, trabecular number, and connectivity density of the femur decreased or remained unchanged, whereas trabecular density and trabecular thickness largely increased. These results suggest a peak in trabecular bone accrual at 8 weeks or earlier followed by further increases in density and structural reorganization of trabeculae. Cortical density, cortical thickness, and cortical cross sectional area increased over time, suggesting a peak in cortical bone accrual at 24 weeks or later. In conclusion, our data provide evidence that growth of muscle lags behind trabecular bone accrual.

ZUSAMMENFASSUNG

Die Muskelkraft wird als eine der wichtigsten Determinanten der Knochenentwicklung betrachtet. Demzufolge sollte ein Maximum im Muskelwachstum einem Maximum im Knochenwachstum vorangehen. In der vorliegenden Studie wurde die Muskel- und Knochenentwicklung in weiblichen C57BL/6J-, DBA/2JRj- und C3H/HeOuJ-Mäusen analysiert. In 8, 16 und 24 Wochen alten Mäusen wurden die kortikale und trabekuläre Knochenstruktur im Femur und die Gewichte ausgewählter Muskeln untersucht. Ein Maximum im Muskelwachstum liegt, unseren Ergebnissen folgend, bei 24 Wochen oder später, wohingegen ein Maximum im trabekulären Knochenwachstum bereits bei 8 Wochen oder früher erreicht wird. Ein Maximum im kortikalen Knochenwachstum liegt ebenfalls bei 24 Wochen oder später. Zusammenfassend zeigen unsere Ergebnisse, dass ein Maximum im trabekulären Knochenwachstum einem Maximum im Muskelwachstum vorangeht. Zusätzliche mechanische Stimuli oder gegenseitige Beeinflussungen auf genetischer und biochemischer Ebene könnten daher eine möglicherweise unterschätzte Rolle im Verhältnis dieser beiden Gewebe während des Wachstums spielen.

Key Words

Bone microstructure - Inbred mouse strains - Peak bone mass - Peak muscle mass

Schlüsselwörter

Knochenmikrostruktur - Inzucht-Mausstämme - Maximale Knochenmasse - Maximale Muskelmasse

Publikationsverlauf

Artikel online veröffentlicht:
26. Januar 2021

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