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Planta Med 1986; 52(4): 321-323
DOI: 10.1055/s-2007-969165
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© Georg Thieme Verlag Stuttgart · New York

Studies on the Tissue Culture of Stevia rebaudiana and Its Components; (II)1. Induction of Shoot Primordia

H. Miyagawa2 , N. Fujioka2 , H. Kohda2 , K. Yamasaki2 , K. Taniguchi3 , R. Tanaka3
  • 2Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan.
  • 3Laboratory of Plant Chromosome and Gene Stock, Faculty of Science, Hiroshima University, Higashisenda 1-1-89, Naka-ku, Hiroshima 730, Japan.
1 Part I, see Ref. (6).
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Publikationsverlauf

1985

Publikationsdatum:
26. Februar 2007 (online)

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Abstract

Shoot primordia, which were able to propagate vegetatively with a very high rate and to redifferentiate easily to new plants, were induced from shoot tips of Stevia rebaudiana Bertoni on Gamborg B5 medium containing 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA) under light. The propagation of the shoot primordia of Stevia rebaudiana is rapid, and they are highly stable in chromosome number and karyotype. The shoot primordia can propagate at a high rate for a long time without differentiation.

At any time, the shoot primordia readily developed into plantlets with shoots and roots within 2 or 3 weeks in static culture on B5 medium containing 0.02 mg/l BAP and 2% sucrose. The plantlets were transplanted to sterilized soil to grow to normal adult plants.

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