Insulin-Induced Electrochemical Changes in Pleura are Associated with the Location within the Pleural Cavity

Abstract

Aim: We investigated the effects of insulin on the electrophysiology of sheep pleural specimens obtained from the upper and lower parts of the pleural cavity and the insulin receptor abundance in these regions.

Materials and methods: Sheep pleural specimens were obtained from the upper and lower lung lobes and from the 1^st–4^th and 8^th–12^th ribs and were mounted between Ussing chambers. Insulin 10⁻⁷ M was added on the mesothelial surface with Insulin Receptor (IR) inhibitor in some experiments. Trans-mesothelial Resistance (R _TM ) was determined. Immunohistochemistry for the presence of IR differences was performed.

Results: Insulin increased the R _TM of all pleural regions. Higher R _TM increase was demonstrated in lower lobe visceral and in caudal parietal specimens. The R _TM increase demonstrated in caudal parietal had the tendency to be higher than that observed in the lower lobe visceral specimens. IR inhibitor abolished insulin's effect in all regions. Immunostaining was more intense for parietal and for caudal parietal specimens when compared with the visceral and lower lobe visceral specimens.

Conclusion: Insulin induces electrochemical alterations that vary depending on the location of specimens within the pleural cavity which possibly is not correlated with insulin receptors variations.

Key words

insulin - pleura - sheep - topography - variations

References

• 1 Blazer-Yost BL, Cox M, Furlanetto R. Insulin and IGF-I receptor mediated Na+ transport in toad urinary bladders.  Am J Physiol. 1989;  257 612-620
• 2 Blazer-Yost BL, Xuehong L, Helman SI. Hormonal regulation of ENaCs: insulin and aldosterone.  Am J Physiol Cell Physiol. 1998;  274 1373-1379
• 3 Chelliah A, Burge MR. Insulin edema in the twenty-first century: review of the existing literature.  J Invest Med. 2004;  52 104-108
• 4 Deachapunya C, Palmer-Densmore M, O’Grady SM. Insulin stimulates transepithelial sodium transport by activation of a protein phosphatase that increases Na-K ATPase activity in endometrial epithelial cells.  J Gen Physiol. 1999;  114 561-574
• 5 Hammerman MR. Interaction of insulin with the renal proximal tubular cell.  Am J Physiol. 1985;  249 F1-F11
• 6 Hatzoglou CH, Gourgoulianis KI, Hatzoglou A. et al . Rapid effects of 17β-estradiol and progesterone on sheep visceral and parietal pleurae via a nitric oxide pathway.  J Appl Physiol. 2002;  93 752-758
• 7 Hatzoglou CH, Gourgoulianis KI, Molyvdas PA. Effect of SNP, ouabain and amiloride on the electrical potential profile of isolated sheep pleura.  J Appl Physiol. 2001;  90 1565-1569
• 8 Hubmayr RD, Walters BJ, Chevalier PA. et al . Topographic distribution of regional lung volume in anesthetized dogs.  J Appl Physiol. 1983;  54 1048-1056
• 9 Kalambokis GN, Tsatsoulis AA, Tsianos EV. The edematogenic properties of insulin.  Am J Kid Dis. 2004;  44 575-590
• 10 Kouritas VK, Hatzoglou C, Foroulis CN. et al . Human parietal pleura present electrophysiology variations according to location in pleural cavity.  Interact Cardiovasc Thorac Surg. 2008;  7 544-547
• 11 Kouritas VK, Hatzoglou C, Gourgoulianis KI. et al . Pleural electrophysiology variations according to location in pleural cavity.  Interact Cardiovasc Thorac Surg. 2009;  9 391-394
• 12 Kouritas VK, Hatzoglou C, Ioannou M. et al . Insulin alters the permeability of sheep pleura.  Exp Clin Endocrinol Diabetes. 2010a;  118 304-309
• 13 Kouritas VK, Hatzoglou CH, Gourgoulianis KI. et al . Role of electrolytes and glucose in the insulin-induced electrochemical effect in sheep pleura.  Exp Clin Endocrinol Diabetes. 2010b;  118 328-332
• 14 Kouritas VK, Ioannou M, Foroulis CN. et al . Insulin-induced electrophysiology changes in human pleura are mediated via its receptor.  Exp Diabetes Res. 2010c;  853176
• 15 Lai-Fook SJ. Pleural mechanics and fluid exchange.  Physiol Rev. 2004;  84 385-410
• 16 McRoberts JA, Riley NE. Regulation of T84 cell monolayer permeability by insulin-like growth factors.  Am J Physiol. 1992;  262 C207-13
• 17 Miura T, Shimada T, Tanaka K. et al . Lymphatic drainage of carbon particles injected into the pleural cavity of the monkey, as studied by video-assisted thoracoscopy and electron microscopy.  J Thorac Cardiovasc Surg. 2000;  120 437-447
• 18 Negrini D, Mukenge S, Del Fabbro M. et al . Distribution of lymphatics in the parietal pleura.  J Appl Physiol. 1991;  70 1544-1549
• 19 Negrini D, Pistolesi M, Minitati M. et al . Regional protein absorption rates from the pleural cavity in dogs.  J Appl Physiol. 1985;  58 2062-2067
• 20 Pezron I, Mitra R, Pal D. et al . Insulin aggregation and asymmetric transport across human bronchial epithelial cell monolayers (Calu-3).  J Pharm Sci. 2002;  91 1135-1146
• 21 Wang NS. The preformed stomas connecting the pleural cavity and the lymphatics in the parietal pleura.  Am Rev Respir Dis. 1975;  111 12-20
• 22 Wang NS. The regional difference of pleural mesothelial cells in rabbits.  Am Rev Respir. 1974;  110 623-633

Correspondence